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Product name
Anti-Pbx3 antibody
See all Pbx3 products (4) ...
Description
Rabbit polyclonal to Pbx3
Tested applications
IHC-P, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow
Immunogen
A region within synthetic peptide: MDDQSRMLQT LAGVNLAGHS VQGGMALPPP PHGHEGADGD GRKQDIGDIL, corresponding to N terminal amino acids 1-50 of Human Pbx3
MDDQSRMLQTLAGVNLAGHSVQGGMALPPPPHGHEGADGD GRKQDIGDIL
Positive control
HepG2 cell lysate and Human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP40426
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 4 isoforms produced by alternative splicing.
Alternative names
- Homeobox protein PBX3 antibody
- MGC134053 antibody
- OTTHUMP00000064213 antibody
see all
Database links
- Entrez Gene: 539222 Cow
- Entrez Gene: 5090 Human
- Entrez Gene: 18516 Mouse
- Entrez Gene: 311876 Rat
- Omim: 176312 Human
- SwissProt: P40426 Human
- SwissProt: O35317 Mouse
- Unigene: 428027 Human
see all
Anti-Pbx3 antibody images:
Western blot - Pbx3 antibody (ab56239)
Anti-Pbx3 antibody (ab56239) at 2.5 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 47 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - Pbx3 antibody (ab56239)
ab56239 (4µg/ml) staining Human Pbx3 in kidney tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained epithelial cells of the renal tubule. Magnification 400X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pbx3 antibody (ab56239)
ab56239 staining Pbx3 in Human prostate tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Pbx3 antibody (ab56239)
This product has been referenced in:
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB; Human.Read more (PubMed: 19023046) »
See 1 publication for this product
Publishing research using ab56239? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"