Anti-Peptide YY/PYY antibody (ab131246)
Key features and details
- Rabbit polyclonal to Peptide YY/PYY
- Suitable for: IHC-P
- Reacts with: Rat, Human, Common marmoset
- Isotype: IgG
Overview
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Product name
Anti-Peptide YY/PYY antibody
See all Peptide YY/PYY primary antibodies -
Description
Rabbit polyclonal to Peptide YY/PYY -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Common marmoset
Predicted to work with: Mouse, Dog, Pig, Macaque monkey, Chinese hamster -
Immunogen
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Positive control
- This antibody gave a positive signal in Rat normal Pancreas formalin fixed paraffin embedded tissue section.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (5) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. -
Sequence similarities
Belongs to the NPY family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 607156 Dog
- Entrez Gene: 5697 Human
- Entrez Gene: 217212 Mouse
- Entrez Gene: 445018 Pig
- Entrez Gene: 287730 Rat
- Omim: 600781 Human
- SwissProt: P68004 Dog
- SwissProt: P10082 Human
see all -
Alternative names
- GHYY antibody
- MGC19143 antibody
- Peptide tyrosine tyrosine antibody
see all
Images
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IHC image of Peptide YY/PYY staining in mouse pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/6000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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IHC image of Peptide YY/PYY staining in Human colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/8000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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IHC image of Peptide YY/PYY staining in marmoset Jejunum formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/5000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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IHC image of Peptide YY/PYY staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131246, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab131246 has been referenced in 3 publications.
- Kim J et al. Clinicopathologic Impact of Peptide Hormonal Expression in Rectal Neuroendocrine Tumors. Arch Pathol Lab Med N/A:N/A (2022). PubMed: 36191342
- Rodnoi P et al. Neuropeptide Y expression marks partially differentiated ß cells in mice and humans. JCI Insight 2:N/A (2017). ICC/IF ; Mouse . PubMed: 28614797
- Suzuki H & Yamamoto T Amylin-like immunoreactivity in pancreatic X cells of the black-spotted frog Rana (Pelophylax) nigromaculata. Tissue Cell 46:535-9 (2014). IHC-Fr ; Frog . PubMed: 25458814