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Flow Cytometry / FACS - Perforin antibody [B-D48] (ab47224)
![Flow Cytometry / FACS - Perforin antibody [B-D48] (ab47224)](/ps/datasheet/images/ab47224_1.gif)
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Product Name
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Perforin antibody [B-D48]
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See all Perforin antibodies (13)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [B-D48] to Perforin
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Immunogen
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Recombinant human perforin.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Flow Cyt
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 10µl for 106 cells or 100 µl of whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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YT-Indy cell line
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Immunology >> Innate Immunity >> Complement >> Other
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
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Relevance
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Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T cell or NK cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin is involved in the killing mediated by cytotoxic lymphocytes, which represents an important mechanism in the immune defense against tumors and virus infections. Human perforin is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kDa. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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B-D48
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Myeloma
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x63-Ag8.653
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Isotype
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IgG1
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Purity
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Ion Exchange Chromatography
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Perforin antibody [B-D48] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Perforin antibody [B-D48] images, references, products related to ab47224 and other tools.
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Perforin antibody [B-D48] images:
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 Flow Cytometry / FACS - Perforin antibody [B-D48] (ab47224)
ab47224, at a dilution of 10µl for 106 cells, staining Perforin in human YT-Indy cell line.
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Search PubMed (MEDLINE) for references to Perforin
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Perforin antibody [B-D48] - more information
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Customer reviews (feedback) regarding Perforin antibody [B-D48] |
Customer FAQs regarding Perforin antibody [B-D48] |
Protocols for Perforin antibody [B-D48] |
Price and availability of products related to Perforin antibody [B-D48] |
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Perforin antibody [B-D48] - related products:
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Perforin antibody [B-D48] - other tools:
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Contact Abcam with a Technical Enquiry about Perforin antibody [B-D48] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry / FACS - Perforin antibody [B-D48] (ab47224)](/index.html?datasheet=47224#ab47224_1.gif)
