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Anti-Perforin antibody [CB5.4] (FITC) (ab16075)

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Overview

Product name

Anti-Perforin antibody [CB5.4] (FITC)
See all Perforin products (14) ...

Description

Rat monoclonal [CB5.4] to Perforin (FITC)

Conjugation

FITC

Tested applications

ICC, IHC-P, IHC-Fr, IPmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin.

Epitope

Recognizes mouse perforin, region amino acids 402-534.

Positive control

Mouse T cell clone B6.1.

General notes

1 test means: 1µl of a 1mg/ml stock solution were used to stain 200,000 cells in a sample volume of 50µl.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Clonality

Monoclonal

Clone number

CB5.4

Isotype

IgG2a

Applications

Show applications key

Our Abpromise guarantee covers the use of ab16075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.

Involvement in disease

Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.

Sequence similarities

Belongs to the complement C6/C7/C8/C9 family.
Contains 1 C2 domain.
Contains 1 EGF-like domain.
Contains 1 MACPF domain.

Domain

The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.

Post-translational
modifications

N-glycosylated.

Cellular localization

Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.

Target information above from: UniProt accessionP14222 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cytolysin antibody
  • FLH2 antibody
  • HPLH2 antibody
  • Lymphocyte pore forming protein antibody
  • Lymphocyte pore-forming protein antibody
  • MGC65093 antibody
  • P1 antibody
  • PERF_HUMAN antibody
  • Perforin 1 antibody
  • Perforin 1 precursor antibody
  • Perforin 1 preforming protein antibody
  • Perforin-1 antibody
  • PFP antibody
  • Pore forming protein antibody
  • PRF 1 antibody
  • PRF1 antibody
see all

References for Anti-Perforin antibody [CB5.4] (FITC) (ab16075)

This product has been referenced in:

  • Dalton JEet al. Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells. J Immunol 173:3660-7 (2004).Read more (PubMed: 15356111) »
  • Crider-Pirkle Set al. Cubilin, a binding partner for galectin-3 in the murine utero-placental complex. J Biol Chem 277:15904-12 (2002).Read more (PubMed: 11856751) »

See all 4 publications for this product

Publishing research using ab16075? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"