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Product Name
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Perforin antibody [CB5.4]
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See all Perforin antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [CB5.4] to Perforin
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Immunogen
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Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin.
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Reacts with
(species key)
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Ms
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Epitope
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This antibody recognizes mouse perforin, region amino acids 402-534.
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Tested applications
(see key)
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ICC, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 5 µg/ml.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa) representing perforin in the mouse T cell clone B6.1.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Immunology >> Innate Immunity >> Complement >> Other
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
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Relevance
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Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T cell or NK cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin is involved in the killing mediated by cytotoxic lymphocytes, which represents an important mechanism in the immune defense against tumors and virus infections. Human perforin is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kDa. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.
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Perforin antibody [CB5.4] (ab16074) |
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 18646 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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CB5.4
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Isotype
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IgG2a
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Perforin antibody [CB5.4] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Perforin antibody [CB5.4] (ab16074)
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This product has been used in: (two most recent references)
Meuth SG et al. Cytotoxic CD8+ T cell-neuron interactions: perforin-dependent electrical silencing precedes but is not causally linked to neuronal cell death. J Neurosci 29:15397-409 (2009). ICC/IF; Mouse. PubMed: 20007464
Dalton JE et al. Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells. J Immunol 173:3660-7 (2004). PubMed: 15356111
See all 5 publication references for this product.
If you publish research using ab16074 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Perforin
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