Anti-Pericentrin antibody - Centrosome Marker (ab4448)
Key features and details
- Rabbit polyclonal to Pericentrin - Centrosome Marker
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-Pericentrin antibody - Centrosome Marker
See all Pericentrin primary antibodies -
Description
Rabbit polyclonal to Pericentrin - Centrosome Marker -
Host species
Rabbit -
Specificity
This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2). -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, African green monkey -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human coronary artery endothelial, MCF7, HeLa and NIH/3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (30) |
Use a concentration of 0.1 - 0.5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.1 - 0.5 µg/ml. |
Target
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Function
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
Tissue specificity
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
Involvement in disease
Microcephalic osteodysplastic primordial dwarfism 2 -
Domain
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
Cellular localization
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 5116 Human
- Entrez Gene: 18541 Mouse
- Omim: 605925 Human
- SwissProt: O95613 Human
- SwissProt: P48725 Mouse
- Unigene: 474069 Human
- Unigene: 251794 Mouse
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Alternative names
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
Images
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ab4448 staining Pericentrin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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ab4448 staining Pericentrin in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.
Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).
Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.
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IF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.
The top panel is an interphase cell showing centrosome staining.
The bottom panel shows a mitotic cell with spindle pole staining.
ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).
Top panel - 630X magnification; Bottom panel -1000X magnification.
The secondary antibody was Alexa-Fluor®488 anti-rabbit.
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NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.
The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.
Datasheets and documents
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SDS download
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Datasheet download
References (487)
ab4448 has been referenced in 487 publications.
- Yue Y et al. Hedgehog-induced ciliary trafficking of kinesin-4 motor KIF7 requires intraflagellar transport but not KIF7's microtubule binding. Mol Biol Cell 33:br1 (2022). PubMed: 34705483
- Lagadec F et al. CRM1 Promotes Capsid Disassembly and Nuclear Envelope Translocation of Adenovirus Independently of Its Export Function. J Virol 96:e0127321 (2022). PubMed: 34757845
- Shen XL et al. LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole. J Cell Biol 221:N/A (2022). PubMed: 34813648
- Tischer T et al. The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly. J Cell Sci 135:N/A (2022). PubMed: 34878135
- Hibino E et al. Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity. BMC Biol 20:42 (2022). PubMed: 35144600