Anti-Pericentrin antibody (ab4448)

  Immunocytochemistry/ Immunofluorescence - Pericentrin antibody - Centrosome Marker (ab4448)

ICC/IF image of ab4448 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4448, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  Immunofluorescence - Anti-Pericentrin antibody (ab4448)

IF staining of pericentrin in MCF7 cells (human).

The top panel is an interphase cell showing centrosome staining.  The bottom panel shows a mitotic cell with spindle pole staining.  ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).

Top panel - 630X magnification; Bottom panel -1000X magnification. The secondary antibody was Alexa488 anti-rabbit.

Gordon Chan, University of Alberta

  Immunocytochemistry/ Immunofluorescence - Pericentrin antibody - Centrosome Marker (ab4448)

NIH3T3 cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature. The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.

Roberto Giambruno, Marilena Ciciarello and Patrizia Lavia

  Immunohistochemistry (Paraffin-embedded sections) - Pericentrin antibody (ab4448)

Image courtesy of Human Protein Atlas

    Paraffin embedded human kidney tissue sections were incubated with ab4448 (1/4500 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab4448 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org

  Immunocytochemistry/ Immunofluorescence - Pericentrin antibody (ab4448)

ICC/IF image of ab4448 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.