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Products:Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Overview
Product name
Anti-Perilipin A+B antibody
See all Perilipin A+B products (2) ...
Description
Rabbit polyclonal to Perilipin A+B
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig
Immunogen
Synthetic peptide: MEPVVRRLST QFTAANELAC RGLDHLEEKI PALQYPPEKI ASELKDTIST, corresponding to a region within N terminal amino acids 73-122 of Human Perilipin A+B
MEPVVRRLST QFTAANELAC RGLDHLEEKI PALQYPPEKI ASELKDTIST
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - Perilipin A+B antibody (ab66514)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay:1:312500
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
Cellular localization
Lipid droplet
Alternative names
- Lipid droplet associated protein antibody
- PERIA antibody
- PERIB antibody
see all
Database links
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
- Unigene: 103253 Human
Anti-Perilipin A+B antibody images:
Western blot - Perilipin A+B antibody (ab66514)
Anti-Perilipin A+B antibody (ab66514) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 56 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
References for Anti-Perilipin A+B antibody (ab66514)
ab66514 has not yet been referenced specifically in any publications.
Publishing research using ab66514? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"