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Products:Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Overview
Product name
Anti-Perilipin A antibody - Carboxyterminal end
See all Perilipin A products (6) ...
Description
Goat polyclonal to Perilipin A - Carboxyterminal end
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Dog
Immunogen
Peptide with sequence C-EPILGRAQYSQLRKK, from the C Terminus human Perilipin A.
EPILGRAQYS QLRKK
Positive control
Human adipose lysate; differentiated 3T3-L1 adipocytes
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.01 - 0.03 µg/ml.Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P: 1/20Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
Tissue specificity
Adipocytes.
Sequence similarities
Belongs to the perilipin family.
Post-translational
modifications
Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
Cellular localization
Lipid droplet. Lipid droplet surface-associated.
Target information above from: UniProt accessionO60240
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
Database links
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
- Unigene: 103253 Human
see all
Anti-Perilipin A antibody - Carboxyterminal end images:
Western blot - Perilipin A antibody (ab61682)
Anti-Perilipin A antibody - Carboxyterminal end (ab61682) at 0.01 µg/ml + Human Adipose lysate at 35 µg
Predicted band size : 56 kDa
Observed band size : 56 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Perilipin A antibody - Carboxyterminal end (ab61682)
Small lipid droplets preferentially stained in the periphery of differentiated 3T3-L1 adipocytesby ab61682 at a dilution of 1ug/ml.
References for Anti-Perilipin A antibody - Carboxyterminal end (ab61682)
This product has been referenced in:
- Yang Xet al. Relative Contribution of Adipose Triglyceride Lipase and Hormone-sensitive Lipase to Tumor Necrosis Factor-a (TNF-a)-induced Lipolysis in Adipocytes. J Biol Chem 286:40477-85 (2011). WB; Mouse.Read more (PubMed: 21969372) »
- Yang Xet al. Identification of a novel splicing isoform of murine CGI-58. FEBS Lett 584:903-10 (2010). ICC/IF; Human.Read more (PubMed: 20083112) »
See all 3 publications for this product
Publishing research using ab61682? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"