Perilipin A antibody (ab3526)

Product Name 

Perilipin A antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Perilipin A

Immunogen 

Synthetic Peptide: CE(502)PIL GRTQYSQLRK KS(517).Immunizi peptide corresponds to amino acid residues 502-517 from rat Perilipin A.
This sequence is 93% conserved in humans.
BLAST 'C-EPILGRTQYSQLRKKS' with or

Reacts with 

Ms

Predicted to react 
(species key)

Hu, Rat
(Due to sequence homology)

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IHC (PFA fixed): Use at an assay dependent concentration (PMID 20049087).
Western Blot: 1/1000.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects an ~62 kDa protein representing Perilipin A from 3T3-L1 cell extract

Cellular localization 

Lipid droplet. Lipid droplet surface-associated

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein

Relevance 

Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids.

Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and Perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, Perilipin is phosphorylated by protein kinase A. Once activated, Perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body.
Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Perilipin A antibody images, references, products related to ab3526 and other tools.

Perilipin A antibody images:

References for Perilipin A antibody (ab3526)

  This product has been used in: (two most recent references)

Rossi CA et al. Clonal characterization of rat muscle satellite cells: proliferation, metabolism and differentiation define an intrinsic heterogeneity. PLoS One 5:e8523 (2010). IHC (PFA fixed); Rat. PubMed: 20049087

Harmer SC et al. Evidence of a possible role for Lys-gamma3-MSH in the regulation of adipocyte function. J Endocrinol 196:149-58 (2008). WB; Mouse. PubMed: 18180326
If you publish research using ab3526 please let us know so that we can cite the reference on this datasheet.

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