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Products:Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Overview
Product name
Anti-Perilipin A antibody
See all Perilipin A products (6) ...
Description
Rabbit polyclonal to Perilipin A
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: PKRRVSDSFFRPSV conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 492-505 of Human Perilipin A
PKRRVSDSFF RPSV
Positive control
Differentiated mouse NIH-3T3-L1 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab50291 is affinity-purified using the immunizing peptide immobilized on agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ICC/IF
Application notes
ICC/IF: Use at a concentration of 5 - 10 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 58 kDa. Detection of the perilipin A band by immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
Tissue specificity
Adipocytes.
Sequence similarities
Belongs to the perilipin family.
Post-translational
modifications
Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
Cellular localization
Lipid droplet. Lipid droplet surface-associated.
Target information above from: UniProt accessionO60240
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
Database links
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
- Unigene: 103253 Human
see all
References for Anti-Perilipin A antibody (ab50291)
This product has been referenced in:
- Thrush ABet al. Skeletal muscle lipogenic protein expression is not different between lean and obese individuals: a potential factor in ceramide accumulation. J Clin Endocrinol Metab 94:5053-61 (2009). WB; Human.Read more (PubMed: 19837942) »
See 1 publication for this product
Publishing research using ab50291? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"