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Anti-Perilipin A antibody (ab60269)

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Overview

Product name

Anti-Perilipin A antibody
See all Perilipin A products (6) ...

Description

Goat polyclonal to Perilipin A

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Pig, Chimpanzee, Rhesus monkey

Immunogen

Synthetic peptide: C-PREKPKRRVSDS, corresponding to iamino acids 488-499 of Human Perilipin

C-PREKPKRR VSDS

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human Adipose tissue lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein

  • Western blot - Perilipin A antibody (ab60269)Western blot - Perilipin A antibody (ab60269) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab60269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.01 µg/mlDetects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).

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    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 20335253)

Target

Function

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.

Tissue specificity

Adipocytes.

Sequence similarities

Belongs to the perilipin family.

Post-translational
modifications

Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.

Cellular localization

Lipid droplet. Lipid droplet surface-associated.

Target information above from: UniProt accessionO60240 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Lipid droplet associated protein antibody
  • Lipid droplet-associated protein antibody
  • PERI antibody
  • Perilipin antibody
  • Perilipin-1 antibody
  • PerilipinA antibody
  • PLIN antibody
  • PLIN1 antibody
  • PLIN1_HUMAN antibody
see all

Database links

see all

Anti-Perilipin A antibody images:

  Western blot - Perilipin A antibody (ab60269)

Western blot - Perilipin A antibody (ab60269)

ab60269 antibody (0.01µg/ml) staining of Perilipin A in Human adipose lysate (35µg protein in RIPA buffer)

Predicted band size : 56 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-Perilipin A antibody (ab60269)

This product has been referenced in:

  • Cheung Wet al. Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication. J Virol 84:6782-98 (2010). ICC/IF; Rhesus monkey.Read more (PubMed: 20335253) »

See 1 publication for this product

Publishing research using ab60269? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"