Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Periostin antibody
See all Periostin products (5) ...
Description
Rabbit polyclonal to Periostin
Tested applications
ICC/IF, IHC-Fr, IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Immunogen
Recombinant fusion protein (Human) produced in E. coli. (75 kDa, 648 amino acid residues of the protein 22-669 and 23 additional amino acid residues).
Properties
Form
Lyophilised:
Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.1M Sodium chloride, 0.05M PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Cardiomyocyte precursors
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
ELISA
Application notes
ELISA: 1/100 - 1/5000.
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/100 - 1/5000.
IHC-Fr: 1/8000 (PMID 19079190).
WB: Use at a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains.
Post-translational
modifications
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ15063
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC119510 antibody
- MGC119511 antibody
- OSF 2 antibody
see all
Database links
- Entrez Gene: 395429 Chicken
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Entrez Gene: 361945 Rat
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
see all
Anti-Periostin antibody images:
Immunohistochemistry (Frozen sections) - Periostin antibody (ab14041)
ab14041 at a 1/100 dilution staining Periostin in mouse dermis tissue by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized using 0.1% Triton. Blocked using 20% serum for 1 hour at 4°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.
This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence-Periostin antibody(ab14041)
ICC/IF image of ab14041 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Periostin antibody (ab14041)
This product has been referenced in:
- Bozyk PD et al. Neonatal periostin knockout mice are protected from hyperoxia-induced alveolar simplication. PLoS One 7:e31336 (2012). WB, IHC-P; Mouse.Read more (PubMed: 22363622) »
- Todorovic V et al. Long form of latent TGF-ß binding protein 1 (Ltbp1L) regulates cardiac valve development. Dev Dyn 240:176-87 (2011). IHC-P; Mouse.Read more (PubMed: 21181942) »
See all 23 publications for this product
Publishing research using ab14041? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"