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Western blot - Peroxiredoxin 1 antibody (ab41906)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 1 antibody (ab41906)
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Product Name
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Peroxiredoxin 1 antibody
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See all Peroxiredoxin 1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Peroxiredoxin 1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Peroxiredoxin 1. (Peptide available as ab41919.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Cow
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following human whole cell lysates: HeLa, A431, Jurkat, HEK293, HepG2, MCF7, SHSY-5Y, U20S
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Cellular localization
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Cytoplasmic
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Research areas
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Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
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Relevance
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Peroxiredoxin 1 is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. It is constitutively expressed in most human cells; is induced to higher levels upon serum stimulation in untransformed and transformed cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.70 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Peroxiredoxin 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Peroxiredoxin 1 antibody images, references, products related to ab41906 and other tools.
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Peroxiredoxin 1 antibody images:
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 Western blot - Peroxiredoxin 1 antibody (ab41906)
All lanes : Peroxiredoxin 1 antibody (ab41906) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 1 antibody (ab41906)
IHC image of Peroxiredoxin 1 staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41906, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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References for Peroxiredoxin 1 antibody (ab41906)
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This product has been used in: (two most recent references)
Cao J et al. Prdx1 inhibits tumorigenesis via regulating PTEN/AKT activity. EMBO J : (2009). WB; Human. PubMed: 19369943
Kumar V et al. Reversible oxidation of mitochondrial peroxiredoxin 3 in mouse heart subjected to ischemia and reperfusion. FEBS Lett 583:997-1000 (2009). WB; Mouse. PubMed: 19230834
If you publish research using ab41906 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Peroxiredoxin 1
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Peroxiredoxin 1 antibody - more information
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