Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Overview
Product name
Anti-Peroxiredoxin 1 antibody
See all Peroxiredoxin 1 products (11) ...
Description
Rabbit polyclonal to Peroxiredoxin 1
Tested applications
ICC/IF, IHC-P, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Peroxiredoxin 1.
(Peptide available as ab41919.)
Positive control
This antibody gave a positive signal in the following human whole cell lysates: HeLa, A431, Jurkat, HEK293, HepG2, MCF7, SHSY-5Y, U20S
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 20631257)
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent concentration.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
Target
Function
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
Post-translational
modifications
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionQ06830
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Hbp23 antibody
- Heme binding 23 kDa protein antibody
- Macrophage 23 Kd stress protein antibody
see all
Database links
- Entrez Gene: 281997 Cow
- Entrez Gene: 5052 Human
- Entrez Gene: 18477 Mouse
- Entrez Gene: 117254 Rat
- Omim: 176763 Human
- SwissProt: Q06830 Human
- SwissProt: P35700 Mouse
- SwissProt: Q63716 Rat
see all
Anti-Peroxiredoxin 1 antibody images:
Western blot - Peroxiredoxin 1 antibody (ab41906)
All lanes : Anti-Peroxiredoxin 1 antibody (ab41906) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 1 antibody (ab41906)
IHC image of Peroxiredoxin 1 staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41906, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-Peroxiredoxin 1 antibody (ab41906)
Anti-Peroxiredoxin 1 antibody (ab41906) at 1 µg/ml + Peroxiredoxin 1 protein (Active) (ab79945) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.
References for Anti-Peroxiredoxin 1 antibody (ab41906)
This product has been referenced in:
- O'Flaherty C & de Souza AR Hydrogen peroxide modifies human sperm peroxiredoxins in a dose-dependent manner. Biol Reprod 84:238-47 (2011). WB; Human.Read more (PubMed: 20864641) »
- Spiess PCet al. Proteomic profiling of acrolein adducts in human lung epithelial cells. J Proteomics 74:2380-94 (2011). Human.Read more (PubMed: 21704744) »
See all 6 publications for this product
Publishing research using ab41906? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"