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Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)
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Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)
(enlarge)
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Product Name
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Peroxiredoxin 1 antibody
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See all Peroxiredoxin 1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Peroxiredoxin 1
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Immunogen
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Synthetic peptide: PLVSDPKRTIAQDY, corresponding to amino acids 103-116 of Human Peroxiredoxin 1 conjugated to diphtheria toxin.
BLAST 'PLVSDPKRTIAQDY' with  or 
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Reacts with
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Hu, Rat
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Predicted to react
(species key)
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Ms, ChHm, Cow, CynMk
(Due to sequence homology)
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Specificity
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ab59538 reacts with Peroxiredoxin 1.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000 - 1/4000.
ICC/IF: 1/50 - 1/100.
IHC-Fr: 1/500 - 1/1000.
IHC-P: 1/500 - 1/1000.
WB: 1/1000 - 1/4000. Predicted molecular weight: 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat kidney and bronchiolar tissues.
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Cellular localization
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Cytoplasmic
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Research areas
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Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
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Relevance
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Peroxiredoxin 1 is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. It is constitutively expressed in most human cells; is induced to higher levels upon serum stimulation in untransformed and transformed cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Peroxiredoxin 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Peroxiredoxin 1 antibody images, references, products related to ab59538 and other tools.
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Peroxiredoxin 1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)
ab59538 at 1/100 dilution staining Peroxiredoxin 1 in Non-ciliated bronchiolar cells (Clara cells) of rat airway by Immunofluorescence. Secondary antibody Jackson Cy3
(1/200).
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Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)
ab59528 at 1/100 dilution staining Peroxiredoxin 1 in Rat kidney tubules by Immunofluorescence. Secondary
antibody Jackson Cy3 (1/200).
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References for Peroxiredoxin 1 antibody (ab59538)
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This product has been used in:
Minamide LS et al. Isolation and characterization of cytoplasmic cofilin-actin rods. J Biol Chem 285:5450-60 (2010). ICC/IF. PubMed: 20022956
If you publish research using ab59538 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Peroxiredoxin 1
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Peroxiredoxin 1 antibody - more information
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Customer reviews (feedback) regarding Peroxiredoxin 1 antibody |
Customer FAQs regarding Peroxiredoxin 1 antibody |
Protocols for Peroxiredoxin 1 antibody |
Price and availability of products related to Peroxiredoxin 1 antibody |
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Peroxiredoxin 1 antibody - related products:
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Peroxiredoxin 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Peroxiredoxin 1 antibody |
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