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Read our guarantee »Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Anti-Peroxiredoxin 1 antibody
See all Peroxiredoxin 1 products (11) ...
Rabbit polyclonal to Peroxiredoxin 1
ab59538 reacts with Peroxiredoxin 1.
WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
Reacts with
Rat, Human
Predicted to work with
Mouse, Cow, Cynomolgus Monkey, Chinese Hamster
Synthetic peptide: PLVSDPKRTIAQDY, corresponding to amino acids 103-116 of Human Peroxiredoxin 1 conjugated to diphtheria toxin.
PLVSDPKRTI AQDY
Rat kidney and bronchiolar tissues.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum
Whole antiserum
Polyclonal
IgG
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
Our Abpromise guarantee covers the use of ab59538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/4000.Predicted molecular weight: 22 kDa.
ELISA: 1/1000 - 1/4000.
IHC-Fr: 1/500 - 1/1000.
IHC-P: 1/500 - 1/1000.
ICC/IF: 1/50 - 1/100.
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionQ06830
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)

ab59538 at 1/100 dilution staining Peroxiredoxin 1 in Non-ciliated bronchiolar cells (Clara cells) of rat airway by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)

ab59528 at 1/100 dilution staining Peroxiredoxin 1 in Rat kidney tubules by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
Western blot - Peroxiredoxin 1 antibody (ab59538)

All lanes : Anti-Peroxiredoxin 1 antibody (ab59538) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
This product has been referenced in:
See all 3 publications for this product
Publishing research using ab59538? Please let us know so that we can cite the reference in this datasheet
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ab59538 at 1/100 dilution staining Peroxiredoxin 1 in Non-ciliated bronchiolar cells (Clara cells) of rat airway by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).

ab59528 at 1/100 dilution staining Peroxiredoxin 1 in Rat kidney tubules by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).

All lanes : Anti-Peroxiredoxin 1 antibody (ab59538) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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