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Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Overview
Product name
Anti-Peroxiredoxin 1 antibody
See all Peroxiredoxin 1 products (11) ...
Description
Rabbit polyclonal to Peroxiredoxin 1
Specificity
ab59538 reacts with Peroxiredoxin 1.
Tested applications
WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Cow, Cynomolgus Monkey, Chinese Hamster
Immunogen
Synthetic peptide: PLVSDPKRTIAQDY, corresponding to amino acids 103-116 of Human Peroxiredoxin 1 conjugated to diphtheria toxin.
PLVSDPKRTI AQDY
Positive control
Rat kidney and bronchiolar tissues.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/4000.Predicted molecular weight: 22 kDa.
ELISA
ELISA: 1/1000 - 1/4000.ELISA: 1/1000 - 1/4000.
IHC-Fr: 1/500 - 1/1000.
IHC-P
IHC-P: 1/500 - 1/1000.IHC-P: 1/500 - 1/1000.
ICC/IF: 1/50 - 1/100.
Target
Function
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
Post-translational
modifications
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionQ06830
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Hbp23 antibody
- Heme binding 23 kDa protein antibody
- Macrophage 23 Kd stress protein antibody
see all
Database links
- Entrez Gene: 281997 Cow
- Entrez Gene: 5052 Human
- Entrez Gene: 18477 Mouse
- Entrez Gene: 117254 Rat
- Omim: 176763 Human
- SwissProt: Q9JKY1 Chinese Hamster
- SwissProt: Q06830 Human
- SwissProt: P35700 Mouse
see all
Anti-Peroxiredoxin 1 antibody images:
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)
ab59538 at 1/100 dilution staining Peroxiredoxin 1 in Non-ciliated bronchiolar cells (Clara cells) of rat airway by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody (ab59538)
ab59528 at 1/100 dilution staining Peroxiredoxin 1 in Rat kidney tubules by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
Western blot - Peroxiredoxin 1 antibody (ab59538)
All lanes : Anti-Peroxiredoxin 1 antibody (ab59538) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
References for Anti-Peroxiredoxin 1 antibody (ab59538)
This product has been referenced in:
- O'Neill JS & Reddy AB Circadian clocks in human red blood cells. Nature 469:498-503 (2011). WB; Human.Read more (PubMed: 21270888) »
- O'Dwyer Det al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr; Human.Read more (PubMed: 22125622) »
See all 3 publications for this product
Publishing research using ab59538? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"