Anti-Peroxiredoxin 3 antibody (ab50300)
Overview
- Product nameAnti-Peroxiredoxin 3 antibodySee all Peroxiredoxin 3 primary antibodies ...
- DescriptionRabbit polyclonal to Peroxiredoxin 3
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat - Immunogen
Synthetic peptide: KPSPAASKEYFQKVNQ conjugated to KLH via an N-terminal lysine residue, corresponding to C terminal amino acids 241-256 of Human Peroxiredoxin 3
- Positive controlHeLa cell mitochondria extracts.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 -
Concentration information loading... - PurityIgG fraction
- Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab50300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/4000 - 1/8000. Predicted molecular weight: 28 kDa. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 21887377 |
Target
- FunctionInvolved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
- Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. - Cellular localizationMitochondrion.
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Database links
- Entrez Gene: 10935 Human
- Entrez Gene: 11757 Mouse
- Entrez Gene: 64371 Rat
- Omim: 604769 Human
- SwissProt: P30048 Human
- SwissProt: P20108 Mouse
- SwissProt: Q9Z0V6 Rat
- Unigene: 523302 Human
- Unigene: 29821 Mouse
- Unigene: 2011 Rat
see all
Target information above from: UniProt accession
P30048
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Antioxidant protein 1 antibodyAOP-1 antibodyAOP1 antibody
- HBC189 antibodyMER5 antibodyMGC104387 antibodyMGC24293 antibodymitochondrial antibodyperoxiredoxin 3 antibodyPeroxiredoxin III antibodyPeroxiredoxin-3 antibodyPRDX3 antibodyPRDX3_HUMAN antibodyPRO1748 antibodyProtein MER5 homolog antibodyPRX III antibodyPrx-III antibodyPRX3 antibodySP 22 antibodySP-22 antibodySP22 antibodyThioredoxin dependent peroxide reductase mitochondrial antibodyThioredoxin-dependent peroxide reductase antibody
see all
Anti-Peroxiredoxin 3 antibody images
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Western blot - Peroxiredoxin 3 antibody (ab50300)Lane 1 : Anti-Peroxiredoxin 3 antibody (ab50300) at 1/4000 dilution
Lane 2 : Anti-Peroxiredoxin 3 antibody (ab50300) at 1/8000 dilution
Lane 1 : HeLa cell mitochondrial lysate
Lane 2 : HeLa cell mitochondrial lysate
Secondary
Goat Anti-Rabbit IgG-Peroxidase
Predicted band size : 28 kDa
Observed band size : 28 kDa
References for Anti-Peroxiredoxin 3 antibody (ab50300)
This product has been referenced in:
- Teng AC et al. Identification of a phosphorylation-dependent nuclear localization motif in interferon regulatory factor 2 binding protein 2. PLoS One 6:e24100 (2011). ICC/IF ; Mouse . Read more (PubMed: 21887377) »
