Anti-Peroxiredoxin 4 antibody [1A1] (ab16754)
Key features and details
- Mouse monoclonal [1A1] to Peroxiredoxin 4
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG3
Overview
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Product name
Anti-Peroxiredoxin 4 antibody [1A1]
See all Peroxiredoxin 4 primary antibodies -
Description
Mouse monoclonal [1A1] to Peroxiredoxin 4 -
Host species
Mouse -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant human fragment protein (without secretion leader sequence) purified from E.coli
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Positive control
- HeLa cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
1A1 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Notes |
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ELISA: Use at an assay dependent dilution.
IP: Use at a concentration of 1 - 2 µg/ml of lysate.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10549 Human
- Omim: 606506 Human
- SwissProt: Q13162 Human
- Unigene: 83383 Human
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Alternative names
- Antioxidant enzyme 372 antibody
- Antioxidant enzyme AOE372 antibody
- AOE37 2 antibody
see all
Images
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Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175
All lanes :
Lane 1 : HeLa Cell Lysate
Lane 2 : Precipitated from HeLa Cell Lysate 200ug using ab16754 2ug
Lane 3 : Precipitated from HeLa Cell Lysate 200ug using ab16754 5ug
Lane 4 : Precipitated from PBS using ab16754 5ug -
ab16754 at 1.5
g/ml immunoprecipitating Peroxiredoxin 4 from HeLa cell lysateµ Lane 1: Input
Lane 2: Precipitates
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab16754 has not yet been referenced specifically in any publications.