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Anti-Peroxiredoxin 4 antibody (ab59542)

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1 question for ab59542

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Question 1

Friday 01-February-2008

We see a band below 35 kD in blots of A549 whole cell lysates, which we believe to be peroxiredoxin 4, and also a band just below 80 kD. In nuclear lysates, we see a band at 60 kD which responds to treatment (viral infection).

ANSWER:

  

I received some useful information from the laboratory that developed this antibody:

"In human lung we see two very clean bands, one around 29kD and the other around 60kD. This protein is reported as being synthesised as a 31kD protein and a signal peptide cleaved prior to secretion resulting in a 27kd protein, a couple of papers have shown these two bands. This protein does form a dimer and under non reducing conditions the two proteins 29 and 60kd are clearly visible. This is shown very clearly in a recently published paper (http://www.biochemj.org/bj/imps/abs/BJ20071428.htm). We would suggest the customer have a read of this paper. We would send an image of a blot but one of our researchers wishes to use it in a publication shortly. We see the two bands 29 and 60kD even on reducing gel/blots which probably reflects incomplete reduction. The blots in the paper above are very similar to what we achieve."

They also suggest something you have no doubt already done: Pubmed search for Peroxiredoxin IV (not 4) in the title.

I hope the above information is useful. If you have any questions, please do not hesitate to contact us.

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