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Products:Cell Biology >> Other Antibodies >> Other Antibodies
Overview
Product name
Anti-Peroxiredoxin 4 antibody
See all Peroxiredoxin 4 products (8) ...
Description
Rabbit polyclonal to Peroxiredoxin 4
Specificity
ab59542 reacts with Peroxiredoxin 4.
Tested applications
WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Cow, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: srvsvadhslhlskakisk, corresponding to amino acids 65-83 of Human Peroxiredoxin 4 conjugated to diphtheria toxin.
srvsvadhsl hlskakisk
Positive control
Non-ciliated bronchiolar cells (Clara cells) in rat lung and sebaceous glands in rat skin.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/4000.Predicted molecular weight: 31 kDa.
ELISA
ELISA: 1/1000 - 1/4000.ELISA: 1/1000 - 1/4000.
IHC-Fr
IHC-Fr: 1/500 - 1/1000.IHC-Fr: 1/500 - 1/1000.
IHC-P: 1/500 - 1/1000.
ICC/IF: 1/50 - 1/100.
Target
Function
Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
Cellular localization
Cytoplasm. Secreted.
Target information above from: UniProt accessionQ13162
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antioxidant enzyme 372 antibody
- Antioxidant enzyme AOE372 antibody
- AOE37 2 antibody
see all
Database links
- Entrez Gene: 281999 Cow
- Entrez Gene: 10549 Human
- Entrez Gene: 53381 Mouse
- Entrez Gene: 85274 Rat
- Omim: 606506 Human
- SwissProt: Q9BGI2 Cow
- SwissProt: Q13162 Human
- SwissProt: O08807 Mouse
see all
Anti-Peroxiredoxin 4 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 4 antibody (ab59542)
ab59542 at 1/100 dilution staining Peroxiredoxin 4 in sebaceous glands in rat skin by Immunohistochemistry. Secondary antibody Jackson biotinylated Donkey anti-rabbit (1/2000) followed by Vector ABC kit and visualised with DAB substrate.
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 4 antibody (ab59542)
ab59542 at 1/100 dilution staining Peroxiredoxin 4 in Non-ciliated bronchiolar cells (Clara cells) in rat lung by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
Western blot - Peroxiredoxin 4 antibody (ab59542)
All lanes : Anti-Peroxiredoxin 4 antibody (ab59542) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Additional bands at : 74 kDa,98 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
The band observed at 27 kDa could potentially be a cleaved form of Peroxiredoxin 4 due to the presence of a 39 amino acid signal peptide.
References for Anti-Peroxiredoxin 4 antibody (ab59542)
This product has been referenced in:
- Aon-Bertolino MLet al. Thioredoxin and glutaredoxin system proteins-immunolocalization in the rat central nervous system. Biochim Biophys Acta 1810:93-110 (2011).Read more (PubMed: 20620191) »
- O'Flaherty C & de Souza AR Hydrogen peroxide modifies human sperm peroxiredoxins in a dose-dependent manner. Biol Reprod 84:238-47 (2011). WB; Human.Read more (PubMed: 20864641) »
See all 4 publications for this product
Publishing research using ab59542? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"