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Products:Cell Biology >> Other Antibodies >> Other Antibodies
Overview
Product name
Anti-Peroxiredoxin 4 antibody
See all Peroxiredoxin 4 products (8) ...
Description
Mouse monoclonal to Peroxiredoxin 4
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey
Immunogen
Recombinant fragment with tag: CHFYAGGQVY PGEASRVSVA DHSLHLSKAK ISKPAPYWEG TAVIDGEFKE LKLTDYRGKY LVFFFYPLDF TFVCPTEIIA FGDRLEEFRS INTEVVACSV, corresponding to amino acids 51 - 151 of Human Peroxiredoxin 4
CHFYAGGQVY PGEASRVSVA DHSLHLSKAK ISKPAPYWEG TAVIDGEFKE LKLTDYRGKY LVFFFYPLDF TFVCPTEIIA FGDRLEEFRS INTEVVACSV
Positive control
Peroxiredoxin 4 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG3
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ELISA: Use at an assay dependent dilution.
Target
Function
Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
Cellular localization
Cytoplasm. Secreted.
Target information above from: UniProt accessionQ13162
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antioxidant enzyme 372 antibody
- Antioxidant enzyme AOE372 antibody
- AOE37 2 antibody
see all
Database links
- Entrez Gene: 281999 Cow
- Entrez Gene: 10549 Human
- Entrez Gene: 53381 Mouse
- Entrez Gene: 85274 Rat
- Omim: 606506 Human
- SwissProt: Q9BGI2 Cow
- SwissProt: Q13162 Human
- SwissProt: O08807 Mouse
see all
Anti-Peroxiredoxin 4 antibody images:
Western blot - Peroxiredoxin 4 antibody (ab68344)
All lanes : Anti-Peroxiredoxin 4 antibody (ab68344) at 1 µg/ml
Lane 1 : Peroxiredoxin 4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 31 kDa
Observed band size : 31 kDa
Western blot - Peroxiredoxin 4 antibody (ab68344)
Predicted band size : 31 kDa
Western blot against tagged recombinant protein immunogen using ab68344 Peroxiredoxin 4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.
References for Anti-Peroxiredoxin 4 antibody (ab68344)
ab68344 has not yet been referenced specifically in any publications.
Publishing research using ab68344? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"