Anti-Peroxiredoxin 6 antibody (ab73350)

Overview

• Product nameAnti-Peroxiredoxin 6 antibodySee all Peroxiredoxin 6 primary antibodies ...
• Description
  Rabbit polyclonal to Peroxiredoxin 6
• Tested applicationsIHC-P, WB, ICC/IF, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Non Human Primates
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Peroxiredoxin 6.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab73681.)

• Positive controlThis antibody gave a positive signal in the following lysates: Testis (Rat) Tissue, Lung (Rat) Tissue, Liver (Mouse) Tissue, Ramos Whole Cell

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Atherosclerosis
  • Ischemia / Reperfusion

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionInvolved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
• Sequence similaritiesBelongs to the ahpC/TSA family. Rehydrin subfamily.
  Contains 1 thioredoxin domain.
• Cellular localizationCytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.

Target information above from: UniProt accession P30041 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9588 Human
  • Entrez Gene: 11758 Mouse
  • Entrez Gene: 94167 Rat
  • Omim: 602316 Human
  • SwissProt: P30041 Human
  • SwissProt: O08709 Mouse
  • SwissProt: O35244 Rat
  • Unigene: 120 Human

  • Unigene: 186185 Mouse
  • Unigene: 42 Rat

  see all

• Alternative names
  1 Cys antibody1 Cys peroxiredoxin antibody1 Cys PRX antibody

  1 cysPrx antibody1-Cys peroxiredoxin antibody1-Cys PRX antibody24 kDa protein antibody9430088D19Rik antibodyAA690119 antibodyAcidic calcium independent phospholipase A2 antibodyacidic calcium-independent phospholipase A2 antibodyaiPLA2 antibodyantioxidant protein 2 antibodyAOP2 antibodyAop2 rs3 antibodyBrp 12 antibodyCiliary body glutathione peroxidase antibodyCP 3 antibodyEC 1.11.1.15 antibodyEC 1.11.1.7 antibodyEC 3.1.1. antibodyGPx antibodyKIAA0106 antibodyLiver 2D page spot 40 antibodyLtw4 antibodyLvtw 4 antibodyMGC46173 antibodymKIAA0106 antibodynon selenium glutathione peroxidase antibodyNon-selenium glutathione peroxidase antibodyNSGPx antibodyORF06 antibodyOTTHUMP00000032693 antibodyp29 antibodyPeroxiredoxin-6 antibodyPeroxiredoxin6 antibodyPHGPx antibodyPhospholipase A2 lysosomal antibodyPLA2 antibodyPRDX 6 antibodyPrdx5 antibodyPRDX6 antibodyPrdx6 rs3 antibodyPRDX6_HUMAN antibodyPRX antibodyRed blood cells page spot 12 antibodyThiol specific antioxidant protein antibody

  see all

Anti-Peroxiredoxin 6 antibody images

• 

  Western blot - Peroxiredoxin 6 antibody (ab73350)
  All lanes : Anti-Peroxiredoxin 6 antibody (ab73350) at 1 µg/ml
  
  Lane 1 : Testis (Rat) Tissue Lysate
  Lane 2 : Lung (Rat) Tissue Lysate
  Lane 3 : Liver (Mouse) Tissue Lysate
  Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 25 kDa
  Observed band size : 25 kDa
  Additional bands at : 48 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 6 antibody (ab73350)
  ICC/IF image of ab73350 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa, HepG2, Hek293 and MCF7 cells at 1µg/ml.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 6 antibody (ab73350)
  IHC image of Peroxiredoxin 6 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73350, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
• 

  Western blot - Anti-Peroxiredoxin 6 antibody (ab73350)
  Anti-Peroxiredoxin 6 antibody (ab73350) at 1 µg/ml + Peroxiredoxin 6 protein (Active) (ab87631) at 0.01 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 30 seconds
• 

  Immunoprecipitation - Anti-Peroxiredoxin 6 antibody (ab73350)
  Peroxiredoxin 6 was immunoprecipitated using 0.5mg Rat Testis tissue lysate, 5µg of Rabbit polyclonal to Peroxiredoxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Rat Testis tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73350.
  Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
  Band: 25KDa: Peroxiredoxin 6