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Overview

  • Product namePeroxiredoxin 6 protein (Active)See all Peroxiredoxin 6 proteins and peptides ...
  • Protein descriptionRecombinant full length Human Peroxiredoxin 6 protein with N- terminal His-tag, amino acids 1-224, MW 27.1kDa (244aa), Swiss-Prot P30041.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurified by using conventional chromatography.
  • Biological activitySpecific activity: approximately 95-120 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute.
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, pH 8
  • Concentration information loading...
  • Sequence notesMGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab87631 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution. Specific activity: approximately 95-120 pmole/min/µg.
    WB WB: Use at an assay dependent concentration. Ab87631 can be used as a WB positive control in conjunction with ab73350.
  • Application notesThis peptide can be used with studies using ab73350 and ab73683.

    • Protein info

    • Alternative names
        1 Cys1 Cys peroxiredoxin1 Cys PRX
        1 cysPrx1-Cys peroxiredoxin1-Cys PRX24 kDa protein9430088D19RikAA690119Acidic calcium independent phospholipase A2Acidic calcium-independent phospholipase A2aiPLA2Antioxidant protein 2AOP2Aop2 rs3Brp 12Ciliary body glutathione peroxidaseCP 3EC 1.11.1.15EC 1.11.1.7EC 3.1.1.GPxKIAA0106Liver 2D page spot 40Ltw4Lvtw 4MGC46173mKIAA0106Non selenium glutathione peroxidaseNon-selenium glutathione peroxidaseNSGPxORF06OTTHUMP00000032693p29Peroxiredoxin-6Peroxiredoxin6PHGPxPhospholipase A2 lysosomalPLA2PRDX 6Prdx5PRDX6Prdx6 rs3PRDX6_HUMANPRXRed blood cells page spot 12Thiol specific antioxidant protein
      see all
  • FunctionInvolved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • Sequence similaritiesBelongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.

    • Target information above from: UniProt accession P30041 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Peroxiredoxin 6 protein (Active) images

    • SDS-PAGE - Peroxiredoxin 6 protein (His tag) (ab87631)
      SDS-PAGE - Peroxiredoxin 6 protein (His tag) (ab87631)
      ab87631 on 15% SDS-PAGE (3µg)
    • Western blot - Anti-Peroxiredoxin 6 antibody (ab73350)
      Western blot - Anti-Peroxiredoxin 6 antibody (ab73350)
      Anti-Peroxiredoxin 6 antibody (ab73350) at 1 µg/ml + Peroxiredoxin 6 protein (Active) (ab87631) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for Peroxiredoxin 6 protein (Active) (ab87631)

    ab87631 has not yet been referenced specifically in any publications.

    Publishing research using ab87631 ? Please let us know so that we can cite the reference in this datasheet

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