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Peroxiredoxin 6 protein (Tagged-His Tag) (ab92263)

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Overview

  • Product namePeroxiredoxin 6 protein (Tagged-His Tag)See all Peroxiredoxin 6 proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-224 of Human Peroxiredoxin 6 with an N terminal His tag; Predicted MWt 26 kDa (UniProt P30041).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 105 µl aqua dest.
  • Storage instructionsStore at 4°C. Upon reconstitution store at -80ºC.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesMPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHP RDFTPVCTT ELGRAAKLAPEFAKRNVKLIALSIDSVED HLAWSKDINAYNCEEPTEKL PFPIIDDRNRELAILLGM LDPAEKDEKGMPVTARVVFVFGPDKKLKLSI LYPATTG RNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIP EE EAKKLFPKGVFTKELPSGKKYLRYTPQP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92263 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        1 Cys1 Cys peroxiredoxin1 Cys PRX
        1 cysPrx1-Cys peroxiredoxin1-Cys PRX24 kDa protein9430088D19RikAA690119Acidic calcium independent phospholipase A2Acidic calcium-independent phospholipase A2aiPLA2Antioxidant protein 2AOP2Aop2 rs3Brp 12Ciliary body glutathione peroxidaseCP 3EC 1.11.1.15EC 1.11.1.7EC 3.1.1.GPxKIAA0106Liver 2D page spot 40Ltw4Lvtw 4MGC46173mKIAA0106Non selenium glutathione peroxidaseNon-selenium glutathione peroxidaseNSGPxORF06OTTHUMP00000032693p29Peroxiredoxin-6Peroxiredoxin6PHGPxPhospholipase A2 lysosomalPLA2PRDX 6Prdx5PRDX6Prdx6 rs3PRDX6_HUMANPRXRed blood cells page spot 12Thiol specific antioxidant protein
      see all
  • FunctionInvolved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • Sequence similaritiesBelongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • Cellular localizationCytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.

    • Target information above from: UniProt accession P30041 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Peroxiredoxin 6 protein (Tagged-His Tag) images

    • SDS-PAGE - Peroxiredoxin 6 protein (Tagged-His Tag) (ab92263)
      SDS-PAGE - Peroxiredoxin 6 protein (Tagged-His Tag) (ab92263)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Peroxiredoxin 6 protein (Tagged-His Tag) (ab92263)

    ab92263 has not yet been referenced specifically in any publications.

    Publishing research using ab92263 ? Please let us know so that we can cite the reference in this datasheet

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