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Overview

  • Product namePertussis toxin protein
  • Protein descriptionFull length native protein (purified) (Bordetella pertussis strain Tohama)
  • Expression hostNative

    • Properties

  • Purification notesab63830 was highly purified (>90% pure) from Bordetella pertussis strain Tohama by the method of Skelton & Wong (Skelton, SK and Wong KH. Simple, efficient purification of filamentous hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic and affinity interaction. J. Clin. Microbiol. 28:1062-1065 (1990) PMID: 2351723).
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: 0.0268% PBS, 10% Glycerol, 0.8% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab63830 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution. Cytotoxicity of the Pertussis Toxin was confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of the Pertussis Toxin.

    Protein info

    • Alternative names
        IAP S1IAP S2IAP S3
        IAP S4IAP S5Islet activating protein S1Islet activating protein S2Islet activating protein S3Islet activating protein S4Islet activating protein S5NAD dependent ADP ribosyltransferasePertussis toxin subunit 1Pertussis toxin subunit 2Pertussis toxin subunit 3Pertussis toxin subunit 4Pertussis toxin subunit 5PTX S1PTX S2PTX S3PTX S4PTX S5PtxAPtxBPtxCPtxDPtxE
      see all
  • RelevancePertussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordetella pertussis. PT catalyzes the ADP-ribosylation of the alpha subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and prevents intracellular signal transduction by preventing the G proteins from interacting with G protein-coupled receptors on the cell membrane. PT consists of one molecule each of S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecules of S4 (12 kDa).
  • Cellular localizationSecreted

    • Pertussis toxin protein images

    • SDS-PAGE - Pertussis toxin protein (ab63830)
      SDS-PAGE - Pertussis toxin protein (ab63830)
      ab63830 on SDS-PAGE.
    • Functional Studies - Pertussis toxin protein (ab63830)
      Functional Studies - Pertussis toxin protein (ab63830)
      Left Panel: Control CHO cell culture without Pertussis Toxin. Right Panel: CHO cells treated with Pertussis Toxin at 0.1 ng/ml in the culture medium for 17 hr at 37oC.

    References for Pertussis toxin protein (ab63830)

    ab63830 has not yet been referenced specifically in any publications.

    Publishing research using ab63830 ? Please let us know so that we can cite the reference in this datasheet

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    Could you tell us, please, in which temperature should ship Pertussis toxin protein (ab63830) http://www.abcam.com/Pertussis-toxin-protein-ab63830.html ?

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    Best regards,

    Thank you for contacting us.

    The protein can be shipped at 4C. However upon arrival it sould be stored at -80C.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More

    Dear technical support: This customer has highly interested in ab63830 (Pertussis toxin protein), this customer wants to know whether this protein was purified from the phase I of Bordetella pertussis strain Tohama or not? Could you please help this custo...

    Read More

    Thank you for your patient. I can confirm the toxin was produced from phase 1 Bordetella pertussis Tohama strain. I hope this information is helpful. Please do not hesitate to contact us if you need further advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"