Overview

  • Product namePhenylalanine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 2 µM
  • Range
    2 µM - 20 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceL-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.
  • Alternative names
    • Phe

Applications

Our Abpromise guarantee covers the use of ab83376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Phenylalanine Assay Kit images

  • Phenylalanine Standard Curve generated following the ab83376 kit protocol.

Protocols

References for Phenylalanine Assay Kit (ab83376)

ab83376 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
The enzyme used in the assay can react with tyrosine and methionine as well as
phenylalanine. Serum methionine concentrations are generally low enough to be
insignificant in this assay. Tyrosine concentrations ...

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Thank youfor your inquiry.

Please excuse the delay in my answer. this kit was not tested in-house and I was in contact with the lab that tested it.

Unfortunately, actual data is proprietary information.

But I was assured t...

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Vielen Dank für Ihren Anruf.

Der im Phenylalanin-AssayKit ab83376verwendete Fluoreszenzfarbstoff (pico-probe) unterliegt zwar demBetriebsgeheimnis; allerdings wird er letztendlich in Resorufin umgewandelt - also genau, wie Sie schon verm...

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Thank you for contacting Abcam. We have not tested if this kit can differentiate between the two forms of phenyl alanine, that does not mean it cannot, it just means that we cannot guaranteed that it will. If there is anything else I can help you with,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"