Phenylalanine Assay Kit (ab83376)
- Product namePhenylalanine Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Sensitivity> 2 µM
- Range2 µM - 20 µM
- Assay time0h 30m
- Product overview
Abcam's Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
Visit our FAQs page for tips and troubleshooting.
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Developer (lyophilized) Red 1 vial Enzyme Mix (lyophilized) Green 1 vial Phenyalanine Standard (10.0 µmol) (lyophilized) Yellow 1 vial Phenylalanine Assay Buffer WM 1 x 25ml Tyrosinase (lyophilized) Blue 1 vial
- Research Areas
Our Abpromise guarantee covers the use of ab83376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Phenylalanine Assay Kit images
Phenylalanine Standard Curve generated following the ab83376 kit protocol.
References for Phenylalanine Assay Kit (ab83376)
ab83376 has not yet been referenced specifically in any publications.