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Phenylalanine Assay Kit (ab83376)

Q&A (4)

Overview

  • Product namePhenylalanine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 2 µM
  • Range
    2 µM - 20 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceL-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.
    • Alternative names
        Phe

    Applications

    Our Abpromise guarantee covers the use of ab83376 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Phenylalanine Assay Kit images

    • Functional Studies - Phenylalanine Assay Kit (ab83376)
      Functional Studies - Phenylalanine Assay Kit (ab83376)
      Phenylalanine Standard Curve generated following the ab83376 kit protocol.

    Protocols

    References for Phenylalanine Assay Kit (ab83376)

    ab83376 has not yet been referenced specifically in any publications.

    Publishing research using ab83376 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 4 of 4 results for Abreviews and Q&A

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    What is the function of Tyrosinase vial provided in the kit?

    Thank you for contacting us.
    The enzyme used in the assay can react with tyrosine and methionine as well as
    phenylalanine. Serum methionine concentrations are generally low enough to be
    insignificant in this assay. Tyrosine concentrations may...

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    Hello,



    We were planning to analyse mouse tissue samples (brain, retina, lung)for phenylalanine content and would be interested to know if you have any further information about the amount of tissue that may be required for use with ...

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    Thank youfor your inquiry.

    Please excuse the delay in my answer. this kit was not tested in-house and I was in contact with the lab that tested it.

    Unfortunately, actual data is proprietary information.

    But I was assured that...

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    Was ist das Fluorochrom?

    Vielen Dank für Ihren Anruf.

    Der im Phenylalanin-AssayKit ab83376verwendete Fluoreszenzfarbstoff (pico-probe) unterliegt zwar demBetriebsgeheimnis; allerdings wird er letztendlich in Resorufin umgewandelt - also genau, wie Sie schon vermute...

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    Well, I am working with an enzyme that is also a methylase that is involved in the N-methylation of a phenylalanine residue. I wonder if I could discriminate a phenylalanine that is methylated from other that is not with your kit?

    Thank you for contacting Abcam. We have not tested if this kit can differentiate between the two forms of phenyl alanine, that does not mean it cannot, it just means that we cannot guaranteed that it will. If there is anything else I can help you with, pl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"