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Abcam's Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
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L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.
|Developer (lyophilized)||Red||1 vial|
|Enzyme Mix (lyophilized)||Green||1 vial|
|Phenyalanine Standard (1.0 µmol) (lyophilized)||Yellow||1 vial|
|Phenylalanine Assay Buffer||WM||1 x 25ml|
|Tyrosinase (lyophilized)||Blue||1 vial|
Our Abpromise guarantee covers the use of ab83376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Phenylalanine measured in cell lysates showing quantities (micromoles) per million of cells tested.
Phenylalanine measured in biofluids showing concentration in micromolar.
Phenylalanine fluorimetric standard curve: mean of duplicates (+/- SD) with background readings subtracted
ab83376 has not yet been referenced specifically in any publications.