Overview

  • Product namePhosphate Assay Kit (Colorimetric)
    See all Phosphate kits
  • Tests
    100 x 1 assay
  • Sample type
    Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeEnzyme activity
  • Sensitivity
    > 1 µM
  • Range
    0.001 mM - 1 mM
  • Assay time
    2h 00m
  • Product overview

    Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonimum molybdate which forms a chromogenic complex with phoshphate ion giving an intense absorption band around OD = 650nm.
    This assay can be used not only in biological fluids but also in inorganic samples such as algal blooms and water from run-off areas of high fertilizer use.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevancePhosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.

Applications

Our Abpromise guarantee covers the use of ab65622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Phosphate Assay Kit (Colorimetric) images

  • Phosphate Standard Curve: Assay was performed following the kit protocol.

Protocols

References for Phosphate Assay Kit (Colorimetric) (ab65622)

This product has been referenced in:

See all 2 Publications for this product

Product Wall



I am very glad to hear that sonification of cells in Tris buffer gave you good results and less background than with a standard RIPA buffer.

I have made a note of this information for our lab as this is extremely useful information.<...

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With this kit ab65622, you can use any generic lysis buffer that you wish for preparing your sample. For example a RIPA buffer or NP-40 buffer is commonly used.

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ab65622 is a colorimetric assay with a working range of 1-10uM concentration of phosphate. ab65622 provides enough materials to carry out 500 assays.

ab102508 is a more sensitive fluorometric...

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The phosphate reagent will only interact with ionic phosphate in your samples, not the ADP and CDP.

Please let me know if you have any further questions.

Hello! I checked with the lab and they confirmed that we have never tried this kit with such samples, however theoretically it will be possible to use them.

Thank you for contacting us.

The kit assays only for the free phosphate. Please make the standards as recommended in water. This is what you are diluting your samples with also.

I hope this information is helpful to you. Please do not...

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Thanks for your query.
I got a reply from our lab regarding your questions:
"The short answer to your question is that you can use mouse plasma and frozen tissues with this assay kit. But there are a few considerations to keep in mind.
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Thanks for the query.

Yes, this kit can be used for your purpose. Use any generic lysis buffer for making the extract. Use a Dounce homogenizer / sonicator for homogenization. Cell and tissue extracts/lysates also contain phosphates from the...

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Thank you for your reply.

I apologize, however there was a misunderstanding. I did not know that you were asking for the malachite green based assay used for assessing the activity of ab78432.

We do not knowwith which kitthe recombi...

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Thank you for your inquiry.

Unfortunately the composition of the buffers of the kit ab65622 is proprietary information. Maybe you can explain why you would like to know more details, and I will be happy to see whether we could help you with o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"