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Overview

  • Product namePhosphate Assay Kit (Colorimetric)See all Phosphate kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Sensitivity
    > 1 µM
  • Range
    0.001 mM - 1 mM
  • Assay time
    2h 00m
  • Product overview

    Abcam's Phosphate Assay Kit (Colorimetric) provides an easy, quick and simple method for assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonimum molybdate which forms a chromogenic complex with phoshphate ion giving an intense absorption band around ? = 650nm.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevancePhosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.

    • Applications

    Our Abpromise guarantee covers the use of ab65622 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Phosphate Assay Kit (Colorimetric) images

    • Functional Studies - Phosphate Colorimetric Assay Kit (ab65622)
      Functional Studies - Phosphate Colorimetric Assay Kit (ab65622)
      Phosphate Standard Curve: Assay was performed following the kit protocol.

    Protocols

    References for Phosphate Assay Kit (Colorimetric) (ab65622)

    This product has been referenced in:
    • Dai H  et al. Transient binding of an activator BH3 domain to the Bak BH3-binding groove initiates Bak oligomerization. J Cell Biol 194:39-48 (2011). Read more (PubMed: 21727192) »

    See 1 Publication for this product

    Publishing research using ab65622 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65622 Submit a Question for ab65622

    Displaying 1 - 9 of 9 results for Abreviews and Q&A

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    Inquiry: Does adenosine 5'-diphosphate, cytidine 5'-diphosphate or inosine 5'-diphosphate react with the phosphate reagent of Kit AB65622? I want to detect only inorganic phosphate as a product from a reaction that uses these nucleotides as sub...

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    Thank you for contacting us.

    The phosphate reagent will only interact with ionic phosphate in your samples, not the ADP and CDP.

    Please let me know if you have any further questions.

    will this kit work with groundwater or rain samples?

    Hello! I checked with the lab and they confirmed that we have never tried this kit with such samples, however theoretically it will be possible to use them.


    Inquiry: Hello, I am a postdoc at U. Southern Calif. I use this phosphate assay kit to detect phosphate from hydrolysis of ATP by Na/K ATPase. I found that when I prepare the standard with just diH2O, it worked well. However, since my samples contain...

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    Thank you for contacting us.

    The kit assays only for the free phosphate. Please make the standards as recommended in water. This is what you are diluting your samples with also.

    I hope this information is helpful to you. Please do not he...

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    I was wondering this this Phosphate Assay Kit (Colorimetric) (ab65622) can be used on mouse plasma (containing EDTA) or mouse tissue (frozen)?

    Thanks for your query.
    I got a reply from our lab regarding your questions:
    "The short answer to your question is that you can use mouse plasma and frozen tissues with this assay kit. But there are a few considerations to keep in mind.
    Read More

    I am a post-doc in Harvard Medical School. My porpose is to check the amount of phosphate uptake in the cells before and after certain treatment. Can I use this kit to for this purpose? What protocol do you recommend? Any special treatment for lysing the ...

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    Thanks for the query.

    Yes, this kit can be used for your purpose. Use any generic lysis buffer for making the extract. Use a Dounce homogenizer / sonicator for homogenization. Cell and tissue extracts/lysates also contain phosphates from the br...

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    With that said, any further details of the assay conditions used to test the recombinant GRP78 activity would be very helpful. I am planning to purchase this item and probably the kit as well for the purpose of conducting activity assays, and your working...

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    Thank you for your reply.

    I apologize, however there was a misunderstanding. I did not know that you were asking for the malachite green based assay used for assessing the activity of ab78432.

    We do not knowwith which kitthe recombinan...

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    Inquiry: Would it be possible for Abcam to post more details of the malachite green-based ATPase activity assay used to evaluate this product? Specifically, the buffer components used would be helpful to know.

    Thank you for your inquiry.

    Unfortunately the composition of the buffers of the kit ab65622 is proprietary information. Maybe you can explain why you would like to know more details, and I will be happy to see whether we could help you with othe...

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    Is this an enzymatic based assay? What reagents are included in the phosphate reagent? Will the kit work with RNA as a sample type?

    Thank you for contacting Abcam regarding ab65622.


    I have consulted with the laboratory and we are not sure about the compatibility of RNA samples. In theory we believe it should work, but cannot say for sure.


    Regarding the assa...

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    Dear technical team,

    Our customer would like to purchase Phosphate Assay Kit and her sample is bacteria.
    I searched ab65622 and would like to know if it is available to her sample.
    http://www.abcam.com/Phosphate-Assay-Kit-Colorimetric...

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    Thank you for your enquiry.

    I can confirm that ab65622 Phosphate Assay Kithas been standardized for mammalian samples.As far as we are aware, it has not used with bacterial samples. Therefore, we regrettably are not able to provide information ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"