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Overview

  • Product namePhosphatidylcholine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 mM
  • Range
    0.2 mM - 2.5 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). Abcam's Phosphatidylcholine Kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevancePhosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.
    • Alternative names
        PC

    Applications

    Our Abpromise guarantee covers the use of ab83377 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Phosphatidylcholine Assay Kit images

    • Functional Studies - Phosphatidylcholine Assay Kit (ab83377)
      Functional Studies - Phosphatidylcholine Assay Kit (ab83377)
      Examples of Colorimetric and Fluorometric Phosphatidylcholine standard curves using ab83377.

    Protocols

    References for Phosphatidylcholine Assay Kit (ab83377)

    ab83377 has not yet been referenced specifically in any publications.

    Publishing research using ab83377 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    Customer kindly contacted us regarding recommended homogenization protocol for tissue culture pellets. Also wondering if there is a detergent in the assay buffer.

    Yes, there is a mild detergent in the assay buffer.
    Here is the tissue homogenization protocol:
    Start with 10-100 mg of the tissue, add 500-1000 μl (or ˜4-6 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passe...

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    Customer kindly contacted us as when they were treating their sample with Hexadecyl phosphocholine (Miltefosine) they saw a marked increase in background. Do we have any knowledge of why this background increase may occur and any ideas of how this can be ...

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    Thank you for contacting us regarding the issue which you have been experiencing when using ab83377.

    Our development team has reviewed this issue and could not find any specific information about interference from this drug. However they sugges...

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    Can you please provide a protocol for preparing cell lysates for use with this Phosphatidylcholine Assay Kit?
    I would like to treat my cells with an enzyme, do you have any recommendations about when in the protocol the treatment can be performed?

    Thank you for your phone call. 
    Any treatment on the cells should be performed before the protocol is begun.  Cell lysates can be prepared using the following protocol:

    Start with 2 x 106 cells and suspend the cell pellet in 500 uL of assa...

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    Hello, I would like to know if the product ab83377 is able to differenciate lysophosphatidylcholine and phosphatidylcholine? Thank you very much for your answer, Best Regards,

    Thank you for your enquiry regarding ab83377. This kit has been tested and characterized for detecting phosphatidylcholine. At this point we do not have such data (reactivity with lysophosphatidylcholine) and this will have to be specifically determined b...

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    Can this Phosphatidylcholine Assay Kit be used on bile samples? Do you have any recommendations for preparing and storing bile samples prior to use with this kit?

    Thank you for contacting us. The lab has not tested this kit for use on bile samples, but it may be suitable. The bile samples can be stored at -80C prior to use and diluted in the provided assay buffer. I hope this information is helpful to you...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"