Overview

  • Product namePhosphatidylcholine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 mM
  • Range
    0.2 mM - 2.5 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). Abcam's Phosphatidylcholine Kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevancePhosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.
  • Alternative names
    • PC

Applications

Our Abpromise guarantee covers the use of ab83377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Phosphatidylcholine Assay Kit images

  • Phosphatidycholine levels measured fluorometrically in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).

  • Phosphatidycholine levels colorimetrically measured  in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

  • Phosphatidycholine levels colorimetrically measured in rat biological fluids (background signal subtracted, mean of duplicates; +/- SD).

  • Examples of Colorimetric and Fluorometric Phosphatidylcholine standard curves using ab83377.

Protocols

References for Phosphatidylcholine Assay Kit (ab83377)

ab83377 has not yet been referenced specifically in any publications.

Product Wall

Yes, there is a mild detergent in the assay buffer.
Here is the tissue homogenization protocol:
Start with 10-100 mg of the tissue, add 500-1000 μl (or ˜4-6 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 pa...

Read More

Thank you for contacting us regarding the issue which you have been experiencing when using ab83377.

Our development team has reviewed this issue and could not find any specific information about interference from this drug. However they sug...

Read More

Thank you for your phone call. 
Any treatment on the cells should be performed before the protocol is begun.  Cell lysates can be prepared using the following protocol:

Start with 2 x 106 cells and suspend the cell pellet in 500 uL of a...

Read More

Thank you for your enquiry regarding ab83377. This kit has been tested and characterized for detecting phosphatidylcholine. At this point we do not have such data (reactivity with lysophosphatidylcholine) and this will have to be specifically determine...

Read More

Thank you for contacting us. The lab has not tested this kit for use on bile samples, but it may be suitable. The bile samples can be stored at -80C prior to use and diluted in the provided assay buffer. I hope this information is helpful to ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"