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Overview

  • Product namePhosphoTracer ERK 1/2 Total ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    100 pg/ml
  • Range
    100 pg/ml - 100000 pg/ml
  • Assay time
    1h 15m
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
    Predicted to work with: Rat
  • Product overview

    PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.

    PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.

    A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.

     

    Abcam's PhosphoTracer ERK 1/2 assay kits detect endogenous levels of ERK 1/2 (GenBank Accessions NP 002737.2 [ERK1], NP 620407 [ERK2]) in cellular lysates. Total ERK 1/2 assay kits detect ERK irrespective of phosphorylation status.



    The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm, a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P28482 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        ERKERK 1ERK-2
        ERK1ERK2ERT1Extracellular signal regulated kinase 1Extracellular signal related kinase 1Extracellular signal-regulated kinase 2HS44KDAPHUMKER1AInsulin stimulated MAP2 kinaseMAP kinase 1MAP kinase 2MAP kinase 3MAP kinase isoform p42MAP kinase isoform p44MAPK 1MAPK 2Mapk1MAPK2MAPK3Microtubule associated protein 2 kinaseMitogen activated protein kinase 3Mitogen-activated protein kinase 1Mitogen-activated protein kinase 2MK01_HUMANp38p40p41p41mapkp42-MAPKP42MAPKp44 ERK1p44 MAPKP44ERK1P44MAPKPRKM1PRKM2PRKM3Protein tyrosine kinase ERK2
      see all
  • Database links
    see all

    • Applications

    Our Abpromise guarantee covers the use of ab119626 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Sandwich ELISA sELISA. Tested in HEK293, HeLa, A431, C2C12, Raw264.7, A549, PC3, NIH3T3, U2OS.

    PhosphoTracer ERK 1/2 Total ELISA Kit images

    • Sandwich ELISA - PhosphoTracer ERK 1/2 Total ELISA kit (ab119626)
      Sandwich ELISA - PhosphoTracer ERK 1/2 Total ELISA kit (ab119626)
      As shown, using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.
    • Sandwich ELISA - PhosphoTracer ERK 1/2 Total ELISA Kit (ab119626)
      Sandwich ELISA - PhosphoTracer ERK 1/2 Total ELISA Kit (ab119626)
      PhosphoTracer ERK 1/2 Total ELISA Kit (ab119626) used in Sandwich ELISA.

    Protocols

    References for PhosphoTracer ERK 1/2 Total ELISA Kit (ab119626)

    ab119626 has not yet been referenced specifically in any publications.

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