PhosphoTracer ERK1/2 pThr202/Tyr204 + Total ELISA Kit (ab119659)
- Product namePhosphoTracer ERK1/2 pThr202/Tyr204 + Total ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture extracts
- Assay typeSandwich
- Assay time1h 15m
- Species reactivityReacts with: Mouse, Hamster, Human
Predicted to work with: Rat
- Product overview
PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam’s PhosphoTracer ERK 1/2 assay kits detect endogenous levels of ERK 1/2 (GenBank Accessions NP_002737.2 [ERK1], NP_620407 [ERK2]) in cellular lysates. The phospho-ERK 1/2 assay detects ERK1 only when phosphorylated at Thr202/Tyr204, and ERK2 only when phosphorylated at Thr185/Tyr187. The total ERK assay detects ERK1/2 irrespective of phosphorylation status.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.
Sensitivity: Phospho-ERK 1/2: 10 pg/ml (Tested with rhERK1), ERK 1/2: 100 pg/ml (Tested with rhERK1).
Range: Phospho-ERK 1/2: 10-10,000 pg/ml (Tested with rhERK1), ERK 1/2: 100-100,000 pg/ml (Tested with rhERK1).
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 96-well PhosphoTracer assay plate (stripwell) 1 unit Adherent plate seal 2 units ADHP (100X) 1 x 120µl ADHP Dilution Buffer 1 x 15ml Assay Control Lysate (lyophilized) 1 x 0.25ml Enhancer Solution 1 x 1ml Lysis Buffer (5X) 1 x 15ml Mouse monoclonal ERK 1/2 (24 assay points) 1 x 0.75ml Mouse monoclonal Phospho-ERK 1/2 (Thr202/Tyr204) (72 assay points) 3 x 0.75ml Rabbit monoclonal ERK 1/2 (HRP) (24 assay points) 1 x 0.75ml Rabbit monoclonal Phospho-ERK 1/2 (HRP) (72 assay points) 3 x 0.75ml Stop Solution 1 x 2ml Wash Buffer (10X) 1 x 15ml
- Research Areas
Contains 1 protein kinase domain.
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
- ERKERK 2ERK-1
- ERK1ERK2ERT1ERT2Extracellular signal regulated kinase 2Extracellular signal-regulated kinase 1HS44KDAPHUMKER1AInsulin-stimulated MAP2 kinaseMAP kinase 1MAP kinase 2MAP kinase 3MAP kinase isoform p42MAP kinase isoform p44MAPK 1MAPK 3MAPK2Mapk3Microtubule-associated protein 2 kinaseMitogen activated protein kinase 2Mitogen-activated protein kinase 1Mitogen-activated protein kinase 3MK03_HUMANp38p40p41p41mapkP42MAPKp44-ERK1p44-MAPKP44ERK1P44MAPKPRKM1PRKM2PRKM3Protein tyrosine kinase ERK2
- Entrez Gene: 5595 Human
- Entrez Gene: 5594 Human
- Entrez Gene: 26417 Mouse
- Entrez Gene: 26413 Mouse
- Entrez Gene: 50689 Rat
- Entrez Gene: 116590 Rat
- Omim: 601795 Human
- Omim: 176948 Human
- SwissProt: P27361 Human
- SwissProt: P28482 Human
- SwissProt: Q63844 Mouse
- SwissProt: P63085 Mouse
- SwissProt: P21708 Rat
- SwissProt: P63086 Rat
- Unigene: 861 Human
Our Abpromise guarantee covers the use of ab119659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||sELISA. Phospho-ERK 1/2: Tested in HeLa, A431, CHO; ERK 1/2: Tested in HEK293, HeLa, A431, C2C12, Raw264.7, A549, PC3, NIH3T3, U2OS.|
PhosphoTracer ERK1/2 pThr202/Tyr204 + Total ELISA Kit images
PhosphoTracer ERK 1/2 (pT202/Y204) + total ERK ELISA Kit (ab119659) used in Sandwich ELISA.
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.
Using Western blot, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.
References for PhosphoTracer ERK1/2 pThr202/Tyr204 + Total ELISA Kit (ab119659)
ab119659 has not yet been referenced specifically in any publications.