PhosphoTracer GAPDH ELISA Kit (ab119627)
- Product namePhosphoTracer GAPDH ELISA Kit
- Detection methodFluorescent
- Tests1 x 96 well plate
- Sample typeCell culture extracts
- Assay typeSandwich
- Sensitivity25 pg/ml
- Range25 pg/ml - 6000 pg/ml
- Assay time1h 15m
- Assay durationOne step assay
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Product overview
PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam's PhosphoTracer GAPDH assay kits detect endogenous levels of GAPDH (GenBank Accession NP_002037) in cellular lysates.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 96-well PhosphoTracer assay plate (stripwell) 1 unit Adherent plate seal 2 units ADHP (100X) 1 x 120µl ADHP Dilution Buffer 1 x 15ml Assay Control Lysate (lyophilized) 1 x 0.25ml Enhancer Solution 1 x 1ml Lysis Buffer (5X) 1 x 15ml Mouse monoclonal GAPDH (HRP) (96 assay points) 1 x 3ml Rabbit monoclonal GAPDH (96 assay points) 1 x 3ml Stop Solution 1 x 2ml Wash Buffer (10X) 1 x 15ml
- Research Areas
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
- Aging associated gene 9 proteinG3P_HUMANG3PD
- GAPDGAPDHGlyceraldehyde 3 phosphate dehydrogenaseGlyceraldehyde-3-phosphate dehydrogenasePeptidyl-cysteine S-nitrosylase GAPDH
Our Abpromise guarantee covers the use of ab119627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||sELISA. Tested in HeLa, A431, A549, PC3, U2OS.|
PhosphoTracer GAPDH ELISA Kit images
As shown, using the GAPDH assay kit, cellular GAPDH is readily detected in A431 cellular lysates, in either untreated cells (-), or cells treated with EGF (+).
Various concentrations of recombinant GAPDH diluted in Lysis Mix containing 0.1% BSA were analyzed using the PhosphoTracer cellular GAPDH assay. The recombinant GAPDH dilutions were transfered to an PhosphoTracer assay plate and analyzed using the standard PhosphoTracer assay protocol. Signal in the wells was determined using a plate reader.
Using the PhosphoTracer GAPDH assay, the amount of lysate required to detect GAPDH in HeLa cell lysates was examined. Up to 50 µg of lysate/well was loaded into replicate wells of an PhosphoTracer microplate, and analyzed for GAPDH. GAPDH was readily detected in less than 1 µg of lysate/well.
References for PhosphoTracer GAPDH ELISA Kit (ab119627)
ab119627 has not yet been referenced specifically in any publications.