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PhosphoTracer JNK 1/2/3 pThr183/Tyr185 + Total ELISA Kit (ab119663)

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Overview

  • Product namePhosphoTracer JNK 1/2/3 pThr183/Tyr185 + Total ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeSandwich
  • Assay time
    1h 15m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.

    PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.

    A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.

     

    Abcam's PhosphoTracer JNKassay kits detect endogenous levels of JNK 1/2/3 (GenBank Accessions NP_620637 [JNK1], NP_620707 [JNK2] and NP_620446 [JNK3]) in cellular lysates. The phospho-JNK assays detects JNK only when phosphorylated at Thr183/Tyr185. The total JNK assay detects JNK irrespective of phosphorylation.



    The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.

  • Notes

    Sensitivity: Phospho-JNK: 50ng/ml (tested with rhJNK1alpha), JNK: 5ng/ml (tested with rhJNK1alpha).

    Range: Phospho-JNK: 50-5000ng/ml (tested with rhJNK1alpha), JNK: 5-5000ng/ml (tested with rhJNK1alpha).

  • Tested applicationsSandwich ELISA more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    96-well PhosphoTracer assay plate (stripwell) 1 unit
    Adherent plate seal 2 units
    ADHP (100X) 1 x 120µl
    ADHP Dilution Buffer 1 x 15ml
    Assay Control Lysate (lyophilized) 1 x 0.25ml
    Enhancer Solution 1 x 1ml
    Lysis Buffer (5X) 1 x 15ml
    Mouse monoclonal JNK 1/2/3 (HRP) (24 assay points) 1 x 0.75ml
    Mouse monoclonal Phospho-JNK 1/2/3 (HRP) (72 assay points) 3 x 0.75ml
    Rabbit monoclonal Phospho-JNK (Thr183/Tyr185)(81E11) (72 assay points) 3 x 0.75ml
    Rabbit polyclonal JNK 1/2/3 (24 assay points) 1 x 0.75ml
    Stop Solution 1 x 2ml
    Wash Buffer (10X) 1 x 15ml
  • Research Areas
  • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

    • Target information above from: UniProt accession P45983 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        c Jun kinase 2c Jun N terminal kinase 2c Jun N terminal kinase 3
        c-Jun N-terminal kinase 1JNK 55JNK-46JNK1JNK1A2JNK2JNK21B1/2JNK2AJNK2ALPHAJNK2BJNK2BETAJNK3JNK3 alpha protein kinaseJNK3AJun kinaseJUN N terminal kinaseMAP kinase 10MAP kinase 8MAP kinase 9MAP kinase p49 3F12MAPK 10MAPK 8MAPK 9MAPK10mapk8MAPK9Mitogen activated protein kinase 10Mitogen activated protein kinase 8 isoform JNK1 alpha1Mitogen activated protein kinase 8 isoform JNK1 beta2Mitogen activated protein kinase 9Mitogen-activated protein kinase 8MK08_HUMANp493F12p54ap54aSAPKp54bSAPKPRKM10PRKM8PRKM9SAPKSAPK1SAPK1aSAPK1bSAPK1cStress activated protein kinase 1aStress activated protein kinase 1bStress activated protein kinase 1cStress activated protein kinase betaStress activated protein kinase JNK2Stress activated protein kinase JNK3Stress-activated protein kinase 1Stress-activated protein kinase JNK1
      see all
  • Database links
    see all

    • Applications

    Our Abpromise guarantee covers the use of ab119663 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Sandwich ELISA sELISA. Phospho-JNK: Tested in HeLa, HEK293, U937, NIH3T3. JNK: Tested in HEK293, HeLa, A431, C2C12, Raw264.7, A549, PC3, NIH3T3, U937.

    PhosphoTracer JNK 1/2/3 pThr183/Tyr185 + Total ELISA Kit images

    • Sandwich ELISA - PhosphoTracer JNK (pT183/Y185) + total JNK ELISA kit (ab119663)
      Sandwich ELISA - PhosphoTracer JNK (pT183/Y185) + total JNK ELISA kit (ab119663)
      Using the JNK 1/2/3 assay kits, a significant stimulation of JNK 1/2/3 phosphorylation at Thr183/Tyr185 is detected in HEK293 cells treated with anisomycin for 30 minutes compared with untreated cells, while no change in total JNK levels is observed.
    • Sandwich ELISA - PhosphoTracer JNK (pT183/Y185) + total JNK ELISA kit (ab119663)
      Sandwich ELISA - PhosphoTracer JNK (pT183/Y185) + total JNK ELISA kit (ab119663)
      A Dilution series of recombinant active JNK1alpha was prepared by diluting to various concentrations in Lysis Mix containing 0.1% BSA. This dilution series was analyzed using the phospho-JNK assay. Limit of detection of active rhJNK was around 50 ng/mL.
    • Sandwich ELISA - PhosphoTracer JNK (pT183/Y185) + total JNK ELISA Kit (ab119663)
      Sandwich ELISA - PhosphoTracer JNK (pT183/Y185) + total JNK ELISA Kit (ab119663)
      PhosphoTracer JNK (pT183/Y185) + total JNK ELISA Kit (ab119663) used in Sandwich ELISA.

    Protocols

    References for PhosphoTracer JNK 1/2/3 pThr183/Tyr185 + Total ELISA Kit (ab119663)

    ab119663 has not yet been referenced specifically in any publications.

    Publishing research using ab119663 ? Please let us know so that we can cite the reference in this datasheet

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    Product code: 119663
    Inquiry: Dear Sirs, I bought an elisa kit of your company: PhosphoTracer JNK 1/2/3 pThr183/Tyr185 + Total ELISA Kit. I'd like to have some information about this kit for CSF analysis. Is it possible to perform?do you have some adv...

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    Thank you for contacting us.

    We have not particularly tested CSF (cerebrospinal fluid?) with this kit however you can use this kit with any human, mouse or rat samples.

    The kit is for qualitative purpose so we may not be able to tell you...

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    I am interested in buying an elisa kit of your company: PhosphoTracer JNK 1/2/3 pThr183/Tyr185 + Total ELISA Kit.

    I'd like to know, if it's possible, how much it costs and in what is phospho tracer JNK different from the usual phospho jnk elisa k...

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    This kit will detect all three JNK form when they are phosphorylated on pThr183/Tyr185. Moreover, it will also detect the whole of the JNK1/2/3.

    The three ELISA kits we have in our catalog differ in the following according to their datashee...

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    Kits phosphotracer
    cibles ERK 1/2, p38 MAPKalpha, JNK 1/2/3.

    Merci de nous avoir contactés.

    Voici la liste des kits

    1) pour la détection d'une seule formed'une protéine particulière :

    ERK 1/2 (pThr202/Tyr204) : ab119625, http://www.abcam.com/ab119625
    ER...

    Read More

    Can this product be used with heart tissue? If so, what type of extraction method and starting concentration is recommended?

    Thank you for contacting Abcam.

    I have talked to the lab about using heart tissue with ab119663 and they have told me that we do not have any data with tissue extracts. However, there should be no reason why this should not work.

    We wo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"