PhosphoTracer SMAD1 (pS463/465) ELISA Kit (ab119647)
Overview
- Product namePhosphoTracer SMAD1 (pS463/465) ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture extracts
- Assay typeSandwich
- Sensitivity5000 cells/well
- Range5000 cells/well - 100000 cells/well
- Assay time1h 15m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat - Product overview
PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam's PhosphoTracer SMAD1 (pSer463/465) assay kits detect endogenous levels of SMAD1 (GenBank Accession NP_001003688) in cellular lysates. Phospho-SMAD1 assay kits only detect SMAD1 when phosphorylated at Ser463/465. Total SMAD1 assay kits detect SMAD1 irrespective of phosphorylation status. Based on sequence similarity, cross reaction to SMAD5 and SMAD8 may occur.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution).
Learn more about the fluorogenic substrate, ADHP. - Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well PhosphoTracer assay plate (stripwell) 1 unit Adherent plate seal 2 units ADHP (100X) 1 x 120µl ADHP Dilution Buffer 1 x 15ml Assay Control Lysate (lyophilized) 1 x 0.25ml Enhancer Solution 1 x 1ml Lysis Buffer (5X) 1 x 15ml Mouse monoclonal SMAD1 (HRP) (96 assay points) 1 x 3ml Phospho SMAD1 (Ser463/465) (96 assay points) 1 x 3ml Stop Solution 1 x 2ml Wash Buffer (10X) 1 x 15ml - Research Areas
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Target information above from: UniProt accession
Q15797
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- BSP-1BSP1HsMAD1
- JV4-1JV41MAD homolog 1MAD mothers against decapentaplegic homolog 1Mad-related protein 1MADH1MADR1Mothers against decapentaplegic homolog 1Mothers against DPP homolog 1SMAD 1SMAD family member 1SMAD mothers against DPP homolog 1Smad1SMAD1_HUMANTGF beta signaling protein 1Transforming growth factor-beta-signaling protein 1
see all
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Entrez Gene: 25671 Rat
- Omim: 601595 Human
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- SwissProt: P97588 Rat
- Unigene: 604588 Human
Applications
Our Abpromise guarantee covers the use of ab119647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA. Tested in HeLa, C2C12. |
PhosphoTracer SMAD1 (pS463/465) ELISA Kit images
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Sandwich ELISA - PhosphoTracer SMAD1 (pS463/465) ELISA Kit (ab119647)Using the SMAD1 assay kits or Western blot, SMAD1 phosphorylation at Ser463/465 is detected in BMP4-treated C2C12 cells (+), compared with C2C12 cells treated with 50 µM dorsomorphin (-). Upon phosphorylation, SMAD1 levels decrease as SMAD1 becomes ubiquitinated. -
Sandwich ELISA - PhosphoTracer SMAD1 (pS463/465) ELISA Kit (ab119647)C2C12 cells were seeded at 5K cells/well in a 96 well tissue culture microplate overnight. The next day cells were serum starved for 60 minutes, then treated with various concentrations of dorsomorphin for 30 mins. Subsequently, the cells stimulated with 50 ng/mL BMP-4 for 30 min. The medium was removed from the wells, and cells were lysed with 120 µl/well of Lysis Mix, with shaking for 10 min. The lysates were transferred to a PhosphoTracer assay plate and assayed for either total or phospho-SMAD1, using the standard protocol. Signal in the wells was determined using a plate reader.
Protocols
References for PhosphoTracer SMAD1 (pS463/465) ELISA Kit (ab119647)
ab119647 has not yet been referenced specifically in any publications.
