PhosphoTracer βCatenin pSer45 + Total ELISA Kit (ab119656)
- Product namePhosphoTracer βCatenin pSer45 + Total ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture extracts
- Assay typeSandwich
- Assay time1h 15m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Product overview
PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam’s PhosphoTracer beta-catenin assay kits detect endogenous levels of beta-catenin (GenBank Accession NP_001895.1) in cellular lysates. Phospho-beta-catenin assays only detect beta-catenin when phosphorylated at Ser45. Total beta-catenin assays detect beta-catenin levels irrespective of phosphorylation.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm, a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.
Sensitivity: Phospho-AKT: 10,000 cells/well (Tested in A549 cells), AKT: 2,000 cells/well (Tested in A549 cells).
Range: Phospho-AKT: 10,000-100,000 cells/well tested (Tested in A549 cells), AKT: 2,000-100,000 cells/well tested (Tested in A549 cells).
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 96-well PhosphoTracer assay plate (stripwell) 1 unit Adherent plate seal 2 units ADHP (100X) 1 x 120µl ADHP Dilution Buffer 1 x 15ml Assay Control Lysate (lyophilized) 1 x 0.25ml Enhancer Solution 1 x 1ml Lysis Buffer (5X) 1 x 15ml Mouse monoclonal beta-Catenin (HRP) (24 assay points) 1 x 0.75ml Mouse monoclonal Phospho-beta-Catenin (HRP) (72 assay points) 3 x 0.75ml Rabbit monoclonal beta-Catenin (24 assay points) 1 x 0.75ml Rabbit polyclonal Phospho-beta-Catenin (Ser45) (72 assay points) 3 x 0.75ml Stop Solution 1 x 2ml Wash Buffer (10X) 1 x 15ml
- Research Areas
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
Contains 12 ARM repeats.
modificationsPhosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
- Beta-cateninBfcCadherin associated protein beta
- Catenin (cadherin associated protein) beta 1Catenin beta-1CatnbCTNB1_HUMANCTNNBCTNNB1Mesc
Our Abpromise guarantee covers the use of ab119656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||sELISA. Phospho-beta-Catenin (S45): Tested in A549, C2C12; beta-Catenin: Tested in HEK293, HeLa, MCF7, A431, A549, PC3, NIH3T3, C2C12|
PhosphoTracer βCatenin pSer45 + Total ELISA Kit images
Using Western blot, beta-catenin phosphorylation at Ser45 is detected in PMA/calyculin-treated A549 cells (+), compared with untreated A549 cells (-).
Using the beta-catenin assay kits, beta-catenin phosphorylation at Ser45 is detected in PMA/calyculin-treated A549 cells (+), compared with untreated A549 cells (-).
PhosphoTracer beta-Catenin (pS45) + total beta-Catenin ELISA Kit (ab119656) used in Sandwich ELISA.
References for PhosphoTracer βCatenin pSer45 + Total ELISA Kit (ab119656)
ab119656 has not yet been referenced specifically in any publications.