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PhosphoTracer p38 MAPKα pThr180/Tyr182 + Total ELISA Kit (ab119665)

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Overview

  • Product namePhosphoTracer p38 MAPKα pThr180/Tyr182 + Total ELISA KitSee all p38 MAPKalpha (phospho Thr180/Tyr182)+ total p38 MAPKalpha kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeSandwich
  • Assay time
    1h 15m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.

    PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.

    A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.

     

    Abcam's PhosphoTracer p38 MAPKalpha assay kits detect endogenous levels of p38 MAPKalpha (GenBank Accession NP_001306) in cellular lysates. The phospho-p38 MAPKalpha assay detects p38 MAPKalpha only when phosphorylated at Thr180/Tyr182. The total p38 MAPKalpha assay detects p38 MAPKalpha irrespective of phosphorylation status.



    The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.

  • Notes

    Sensitivity: Phospho-p38 MAPKalpha: 0.1 ng/ml (tested with rh p38 MAPK), p38 MAPKalpha: 1 ng/ml (tested with rh p38 MAPK).

    Range: Phospho-p38 MAPKalpha: 0.1-250 ng/ml (tested with rh p38 MAPK), p38 MAPKalpha: 1-250 ng/ml (tested with rh p38 MAPK).

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession Q16539 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CSAID binding proteinCSAID-binding proteinCsaids binding protein
        CSBPCSBP1CSBP2CSPB1Cytokine suppressive anti-inflammatory drug-binding proteinEXIPMAP kinase 14MAP kinase MXI2MAP kinase p38 alphaMAPK 14MAPK14MAX-interacting protein 2Mitogen-activated protein kinase 14Mitogen-activated protein kinase p38 alphaMK14_HUMANMxi2p38p38 MAP kinasep38 mitogen activated protein kinasep38alphap38alpha ExipPRKM14PRKM15RKSAPK2AStress activated protein kinase 2A
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119665 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA. Tested in HeLa, THP1, Jurkat.

      PhosphoTracer p38 MAPKα pThr180/Tyr182 + Total ELISA Kit images

      • Sandwich ELISA - PhosphoTracer p38 MAPKalpha (pT180/Y182) + total p38 MAPKalpha ELISA kit (ab119665)
        Sandwich ELISA - PhosphoTracer p38 MAPKalpha (pT180/Y182) + total p38 MAPKalpha ELISA kit (ab119665)
        As shown, using the p38 MAPKalpha assay kits, a significant stimulation of p38 MAPKalpha phosphorylation at Thr180/Tyr182 is detected in HEK293 cells treated with anisomycin for 30 minutes compared with untreated cells, while no change in total p38 MAPKalpha levels is observed.
      • Sandwich ELISA - PhosphoTracer p38 MAPKalpha (pT180/Y182) + total p38 MAPKalpha ELISA kit (ab119665)
        Sandwich ELISA - PhosphoTracer p38 MAPKalpha (pT180/Y182) + total p38 MAPKalpha ELISA kit (ab119665)
        Using the PhosphoTracer phospho-p38 MAPKalpha assay, the length of assay incubation time that was required to detect recombinant phospho-p38 MAPKalpha was examined. After 15 mins, phospho-p38 MAPKalpha concentrations of approximately 4 ng/mL were readily detected, while after 1 hour incubation, the limit of detection was approximately 1 ng/mL, and with a much improved assay window. Incubation times longer than one hour showed a slight improvement on assay performance.
      • Sandwich ELISA - PhosphoTracer p38 MAPKalpha (pT180/Y182) + total p38 MAPKalpha ELISA Kit (ab119665)
        Sandwich ELISA - PhosphoTracer p38 MAPKalpha (pT180/Y182) + total p38 MAPKalpha ELISA Kit (ab119665)
        PhosphoTracer p38 MAPKalpha (pT180/Y182) + total p38 MAPKalpha ELISA Kit (ab119665) used in Sandwich ELISA.

      Protocols

      References for PhosphoTracer p38 MAPKα pThr180/Tyr182 + Total ELISA Kit (ab119665)

      ab119665 has not yet been referenced specifically in any publications.

      Publishing research using ab119665 ? Please let us know so that we can cite the reference in this datasheet

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      Kits phosphotracer
      cibles ERK 1/2, p38 MAPKalpha, JNK 1/2/3.

      Merci de nous avoir contactés.

      Voici la liste des kits

      1) pour la détection d'une seule formed'une protéine particulière :

      ERK 1/2 (pThr202/Tyr204) : ab119625, http://www.abcam.com/ab119625
      ER...

      Read More

      Dear Sir/Madam

      We want to detect MAPKp (MAP kinase phosphatase) by ELISA essay and are looking for ELIZA kit. We have not found it at your website. Do you have some other catalogue to find this kit?

      Sincerely

      Thank you for your enquiry.

      I can confirm we have the following MAP kinase alpha ELISA kits avaiable. I can recommend to review the online datashsheets for further information:

      ab119638 PhosphoTracer p38 MAPKalpha (pT180/Y182) ELISA ...

      Read More

      Dear abcam team,

      Thanks for your reply. Actually, I did some readings and I found that it is necessary to have both the total and phosphorylated proteins quantified. So in principle, I can use the 24 of the total protein assays and 24/72 of the...

      Read More

      Thank you fory our reply.

      I am sorry to confirm we do not have smaller kits. We do have the following separate kits forp38 MAPKalpha (pT180/Y182) and total p38 MAPKalpha, however these are sold as1x 96 tests

      ab119638 PhosphoTracer p3...

      Read More

      Dear Technical Team Service,


      Thanks a lot for your clear reply but still I have some questions:

      * In your opinion, it is not necessary always to test the total protein in attempt to study the phosphorylation status?

      ...

      Read More

      Thank you for your reply.

      In answer to your questions:

      1. It is definitely important to establish that a particular treatment that causes modulation of phosphorylation does not also modulate expression/levels of the protein of intere...

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      Dear abcam Technical Support Team,

      I am interested to use the ab119665 ELISA kit. I have some questions about the kit and the protocol:

      - The kit contains 72 phospho-p38 and 24 total p38. Why there are not equal number of assays for ...

      Read More

      Thank you for your enquiry.

      I am pleased to help answer your questions:

      1. We have packaged the products 72/24 as most customers are interested mainly in the regulation of phosphorylation of any particular target, and will tend to us...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"