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Overview

  • Product namePhosphoTracer p70S6K (pT389) ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    1 ng/ml
  • Range
    1 ng/ml - 300 ng/ml
  • Assay time
    1h 15m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.

    PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.

    A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.

     

    Abcam's PhosphoTracer p70S6K assay kits detect endogenous levels of p70S6K (GenBank Accession NP_003152) in cellular lysates. Phospho-p70S6K assay kits detect p70S6K only when phosphorylated at Thr389. Total p70S6K assay kits detect p70S6K, irrespective of phosphorylation status.



    The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionPhosphorylates specifically ribosomal protein S6.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Target information above from: UniProt accession Q9UBS0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        70 kDa ribosomal protein S6 kinase 170 kDa ribosomal protein S6 kinase 2KLS
        KS6B2_HUMANp70 alphaP70 beta 1P70 beta 2p70 ribosomal S6 kinase betap70 S6 kinase alpha 1p70 S6 kinase alpha 2p70 S6 kinase betap70 S6Kp70 S6K alphap70 S6K-betap70 S6KAp70 S6KBp70(S6K) alphap70(S6K) betap70-betap70-S6K 2P70S6K1P70S6K2p70S6KbPS6KRibosomal protein S6 kinase 70kDa polypeptide 1Ribosomal protein S6 kinase 70kDa polypeptide 2Ribosomal protein S6 kinase beta 1Ribosomal protein S6 kinase beta-2Ribosomal protein S6 kinase IRPS6KB1Rps6kb2S6 kinase-related kinaseS6KS6K beta 1S6K-betaS6K-beta-2S6K1S6K2Serine/threonine kinase 14 alphaSerine/threonine protein kinase 14 betaSerine/threonine protein kinase 14ASerine/threonine-protein kinase 14BSRKSTK14ASTK14B
      see all
  • Database links
    see all

    • Applications

    Our Abpromise guarantee covers the use of ab119642 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Sandwich ELISA sELISA. Tested in MCF7, PC3.

    PhosphoTracer p70S6K (pT389) ELISA Kit images

    • Sandwich ELISA - PhosphoTracer p70S6K (pT389) ELISA kit (ab119642)
      Sandwich ELISA - PhosphoTracer p70S6K (pT389) ELISA kit (ab119642)
      As shown, using the p70S6K assay kits, a significant reduction of p70S6K phosphorylation at Thr389 is detected in MCF7 cells treated with wortmannin for 3 hours, compared with untreated cells, while no change in total p70S6K levels was observed.
    • Sandwich ELISA - PhosphoTracer p70S6K (pT389) ELISA kit (ab119642)
      Sandwich ELISA - PhosphoTracer p70S6K (pT389) ELISA kit (ab119642)
      Using the PhosphoTracer phospho-p70S6K assay, the length of assay incubation time that was required to detect recombinant phospho-p70S6K was examined. After 15 mins, phospho- p70S6K concentrations of approximately 2 ng/mL were readily detected, while after 1 hour incubation, the limit of detection was approximately 0.5 ng/mL. Longer incubation times had little effect on overall limit of detection that was observed.
    • Sandwich ELISA - PhosphoTracer p70S6K (pT389) ELISA Kit (ab119642)
      Sandwich ELISA - PhosphoTracer p70S6K (pT389) ELISA Kit (ab119642)
      PhosphoTracer p70S6K (pT389) ELISA Kit (ab119642) used in Sandwich ELISA.

    Protocols

    References for PhosphoTracer p70S6K (pT389) ELISA Kit (ab119642)

    ab119642 has not yet been referenced specifically in any publications.

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