Recombinant Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1911] to Phospholamban (phospho S16)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Phospholamban (phospho S16) antibody [EPR1911]
See all Phospholamban primary antibodies -
Description
Rabbit monoclonal [EPR1911] to Phospholamban (phospho S16) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal heart lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
This antibody is not purified. It is provided in cell supernatant and storage buffer. -
Clonality
Monoclonal -
Clone number
EPR1911 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: 1/200,000 - 1/500,000. Detects a band of approximately 11 kDa (predicted molecular weight: 6 kDa).
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
Tissue specificity
Heart muscle (at protein level). -
Involvement in disease
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
Sequence similarities
Belongs to the phospholamban family. -
Post-translational
modificationsPhosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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Database links
- Entrez Gene: 5350 Human
- Entrez Gene: 18821 Mouse
- Omim: 172405 Human
- SwissProt: P26678 Human
- SwissProt: P61014 Mouse
- Unigene: 170839 Human
- Unigene: 745010 Human
- Unigene: 34145 Mouse
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Alternative names
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
Images
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All lanes : Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697) at 1/200000 dilution
Lane 1 : Fetal heart lysate (untreated)
Lane 2 : Fetal heart lysate (treated with LP)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 6 kDa
Actual band size is 11 KDa.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab92697 has been referenced in 2 publications.
- Horvath C et al. Pleiotropic, non-cell death-associated effects of inhibitors of receptor-interacting protein kinase 1 in the heart. Mol Cell Biochem 476:3079-3087 (2021). PubMed: 33811579
- Molina CE et al. Profibrotic, Electrical, and Calcium-Handling Remodeling of the Atria in Heart Failure Patients With and Without Atrial Fibrillation. Front Physiol 9:1383 (2018). PubMed: 30356673