RabMAb

Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697)

Overview

  • Product nameAnti-Phospholamban (phospho S16) antibody [EPR1911]
    See all Phospholamban primary antibodies
  • Description
    Rabbit monoclonal [EPR1911] to Phospholamban (phospho S16)
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 16 of Human Phospholamban.

  • Positive control
    • Fetal heart lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: 1/50.
    WB: 1/200,000 - 1/500,000. Detects a band of approximately 11 kDa (predicted molecular weight: 6 kDa).

    Is unsuitable for IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
    • Tissue specificityHeart muscle (at protein level).
    • Involvement in diseaseCardiomyopathy, dilated 1P
      Cardiomyopathy, familial hypertrophic 18
    • Sequence similaritiesBelongs to the phospholamban family.
    • Post-translational
      modifications
      Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
    • Cellular localizationSarcoplasmic reticulum membrane. Mitochondrion membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiac phospholamban antibody
      • CMD1P antibody
      • CMH18 antibody
      • PLB antibody
      • PLN antibody
      • PPLA_HUMAN antibody
      see all

    Anti-Phospholamban (phospho S16) antibody [EPR1911] images

    • All lanes : Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697) at 1/200000 dilution

      Lane 1 : Fetal heart lysate (untreated)
      Lane 2 : Fetal heart lysate (treated with LP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size : 6 kDa

    References for Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697)

    ab92697 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab92697.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"