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Phospholipase A2 IIA protein (His tag) (ab63843)

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Overview

  • Product namePhospholipase A2 IIA protein (His tag)
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 21-144 of Human Phospholipase A2 IIA, with an N-Terminal 16 amino acid His tag. (total 140 amino acids).
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    0.05M Acetate buffer pH 4.0
  • Concentration information loading...
  • Additional notesProduct is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Sequence notesMRGSHHHHHH GMASHMNLVN FHRMIKLTTG KEAALSYGFY GCHCGVGGRG SPKDATDRCC VTHDCCYKRL EKRGCGTKFL SYKFSNSGSR ITCAKQDSCR SQLCECDKAA ATCFARNKTT YNKKYQYYSN KHCRGSTPRC
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab63843 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.

    Protein info

    • Alternative names
        EC 3.1.1.4GIIC sPLA2Group IIA phospholipase A2
        MOM1Non pancreatic secretory phospholipase A2NPS PLA2NPSPLA2Phosphatidylcholine 2 acylhydrolasePhospholipase A2 group IIA (platelets synovial fluid)Phospholipase A2 membrane associated precursorPLA2PLA2BPLA2LPLA2SPLAS1RASF ARASFAsPLA2
      see all
  • RelevancePhospholipase A2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, a precursor of eicosanoids including prostaglandins and leukotrienes. This enzyme has been proposed to hydrolyze phosphatidylcholine in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci. Phospholipase A2 IIA can exert beneficial action in the context of infectious diseases since recent studies have shown that this enzyme exhibits potent bactericidal effects. Induction of the synthesis of Phospholipase A2 IIA is generally initiated by endotoxin and a limited number of cytokines via paracrine and/or autocrine processes.
  • Cellular localizationCell Membrane; peripheral membrane protein

    • Phospholipase A2 IIA protein (His tag) images

    • SDS-PAGE - Phospholipase A2 IIA protein (His tag) (ab63843)
      SDS-PAGE - Phospholipase A2 IIA protein (His tag) (ab63843)
      12% SDS-PAGE separation of ab63843. Molecular Weight: ~15.8 kDa.

      Lane 1. Molecular weight marker
      Lane 2. reduced and heated sample, 10µg/lane
      Lane 3. non-reduced and non-heated sample, 10µg/lane

    References for Phospholipase A2 IIA protein (His tag) (ab63843)

    ab63843 has not yet been referenced specifically in any publications.

    Publishing research using ab63843 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"