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Phospholipase A2 IIE protein (His tag) (ab63845)

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Overview

  • Product namePhospholipase A2 IIE protein (His tag)
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 20-142 of the Human Phospholipase A2 IIE precursor, with a 16 amino acid N-terminal His tag.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    0.05M Acetate buffer pH 4.0
  • Concentration information loading...
  • Sequence notesMRGSHHHHHH GMASHMNLVQ FGVMIEKMTG KSALQYNDYG CYCGIGGSHW PVDQTDWCCH AHDCCYGRLE KLGCEPKLEK YLFSVSERGI FCAGRTTCQR LTCECDKRAA LCFRRNLGTY NRKYAHYPNK LCTGPTPPC
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab63845 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        2IIEEC 3.1.1.4GIIE sPLA2
        Group IIE secretory phospholipase A2Group IIE secretory phospholipase A2 precursorPA2GE_HUMANPhosphatidylcholine 2 acylhydrolase GIIEPhosphatidylcholine 2-acylhydrolase 2EPhospholipase A2 group IIEPla2g2esPLAsPLA2-IIE
      see all
  • FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
  • Tissue specificityRestricted to the brain, heart, lung, and placenta.
  • Sequence similaritiesBelongs to the phospholipase A2 family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession Q9NZK7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Phospholipase A2 IIE protein (His tag) images

    • SDS-PAGE - Phospholipase A2 IIE protein (His tag) (ab63845)
      SDS-PAGE - Phospholipase A2 IIE protein (His tag) (ab63845)
      12% SDS-PAGE separation of ab63845. Molecular Weight: ~15.8 kDa.

      Lane 1. Molecular weight marker
      Lane 2. reduced and heated sample, 5µg/lane
      Lane 3. non-reduced and non-heated sample, 5µg/lane

    References for Phospholipase A2 IIE protein (His tag) (ab63845)

    ab63845 has not yet been referenced specifically in any publications.

    Publishing research using ab63845 ? Please let us know so that we can cite the reference in this datasheet

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