The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
Tissue specificityFound in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
Sequence similaritiesBelongs to the phospholipase A2 family.
Predicted band size : 17 kDa Observed band size : 17 kDa
Image from Curfs DM et al, J Biol Chem. 2008 Aug 1;283(31):21640-8. Epub 2008 May 28, Fig 1.DOI 10.1074/jbc.M710584200
References for Anti-Phospholipase A2 X antibody (ab47111)
This product has been referenced in:
Curfs DM et al. Macrophage Secretory Phospholipase A2 Group X Enhances Anti-inflammatory Responses, Promotes Lipid Accumulation, and Contributes to Aberrant Lung Pathology. J Biol Chem283:21640-8 (2008).
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