Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247)

Overview

• Product nameAnti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y]See all Phospholipase C beta 3 primary antibodies ...
• Description
  Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3 (phospho S537)
• SpecificityThis antibody detects Phospholipase C beta 3 phosphorylated on Ser537.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Phosphor-specific peptide corresponding to residues surrounding Serine 537 of human Phospholipase C beta 3.

• Positive controlHeLa cell lysate.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR1498Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • PLC

Applications

Target

• FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
• Sequence similaritiesContains 1 C2 domain.
  Contains 1 PI-PLC X-box domain.
  Contains 1 PI-PLC Y-box domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession Q01970 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5331 Human
  • Omim: 600230 Human
  • SwissProt: Q01970 Human
  • Unigene: 591953 Human
  • Unigene: 523761 Human

• Alternative names
  1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody1-phosphatidylinositol-4 antibody5-bisphosphate phosphodiesterase beta-3 antibody

  Phosphoinositide phospholipase C antibodyPhosphoinositide phospholipase C-beta-3 antibodyPhospholipase C beta 3 antibodyPhospholipase C beta 3 (phosphatidylinositol specific) antibodyPhospholipase C-beta-3 antibodyPLC beta 3 antibodyPLC-beta-3 antibodyPlcb3 antibodyPLCB3_HUMAN antibody

  see all

Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] images

• 

  Western blot - Phospholipase C beta 3 (phospho S1105) antibody [EPR1498Y] (ab51247)
  All lanes : Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution
  
  Lane 1 : HeLa cell lysate, untreated
  Lane 2 : HeLa cell lysate, treated with TPA
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-rabbit HRP labeled at 1/2000 dilution
  
  Predicted band size : 139 kDa
  Observed band size : 150 kDa (why is the actual band size different from the predicted?)