RabMAb

Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247)

Overview

  • Product nameAnti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y]
    See all Phospholipase C beta 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3 (phospho S537)
  • SpecificityThis antibody detects Phospholipase C beta 3 phosphorylated on Ser537.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phosphor-specific peptide corresponding to residues surrounding Serine 537 of human Phospholipase C beta 3.

  • Positive control
    • HeLa cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab51247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
  • Target

    • FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase beta-3 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-beta-3 antibody
      • Phospholipase C beta 3 antibody
      • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
      • Phospholipase C-beta-3 antibody
      • PLC beta 3 antibody
      • PLC-beta-3 antibody
      • Plcb3 antibody
      • PLCB3_HUMAN antibody
      see all

    Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] images

    • All lanes : Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 139 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    References for Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247)

    ab51247 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab51247.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"