Recombinant Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y]
See all Phospholipase C beta 3/PLCB3 primary antibodies -
Description
Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa whole cell lysate (ab150035).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1498Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa). |
Target
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Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5331 Human
- Omim: 600230 Human
- SwissProt: Q01970 Human
- Unigene: 523761 Human
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Alternative names
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
see all
Images
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All lanes : Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab51247 has not yet been referenced specifically in any publications.