Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Overview
Product name
Anti-Phospholipase C gamma 1 antibody [1F1]
See all Phospholipase C gamma 1 products (18) ...
Description
Mouse monoclonal [1F1] to Phospholipase C gamma 1
Tested applications
ELISA, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length protein (Human).
Positive control
HepG2 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
1F1
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
IP
Application notes
ELISA: Use at an assay dependent dilution.
IP: Use at a concentration of 1 µg/ml lysate.
WB: 1/4000. Predicted molecular weight: 149 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Post-translational
modifications
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.
Ubiquitinated by CBLB in activated T-cells.
Target information above from: UniProt accessionP19174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
see all
Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all
References for Anti-Phospholipase C gamma 1 antibody [1F1] (ab16953)
ab16953 has not yet been referenced specifically in any publications.
Publishing research using ab16953? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"