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Anti-Phospholipase C gamma 1 antibody [2B1] (ab16955)

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    Overview

    Product name

    Anti-Phospholipase C gamma 1 antibody [2B1]
    See all Phospholipase C gamma 1 products (18) ...

    Description

    Mouse monoclonal [2B1] to Phospholipase C gamma 1

    Tested applications

    ELISA, WB, IPmore details

    Cross reactivity

    Reacts with

    Mouse, Rat, Human

    Predicted to work with

    Ferret

    Immunogen

    Recombinant full length protein (Human).

    Positive control

    HepG2 whole cell lysate.

    Properties

    Form

    Liquid

    Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Storage buffer

    Preservative: None
    Constituents: 50% Glycerol, PBS

    Concentration

    Concentration information loading...

    Purity

    IgG fraction

    Clonality

    Monoclonal

    Clone number

    2B1

    Isotype

    IgG2a

    Light chain type

    kappa

    Research areas

    Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
    Signal Transduction >> Metabolism >> Lipid metabolism
    Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
    Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis

    • Western blot - Phospholipase C gamma antibody [2B1] (ab16955)Western blot - Phospholipase C gamma antibody [2B1] (ab16955) image (enlarge)

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab16955 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application notes

    ELISA: Use at an assay dependent dilution.
    IP: Use at a concentration of 1 µg/ml lysate.
    WB: 1/2000. Predicted molecular weight: 149 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    Function

    PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

    Sequence similarities

    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

    Domain

    The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.

    Post-translational
    modifications

    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.
    Ubiquitinated by CBLB in activated T-cells.

    Target information above from: UniProt accessionP19174 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010).

    Information by UniProt

    Alternative names

    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase gamma-1 antibody
    • inositoltrisphosphohydrolase antibody
    • monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • phosphatidylinositol phospholipase C antibody
    • phosphodiesterase gamma 1 antibody
    • phosphoinositidase C antibody
    • phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C 148 antibody
    • phospholipase C gamma 1 (formerly subtype 148) antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C gamma 1 antibody
    • phospholipase C-148 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC 1 antibody
    • PLC 148 antibody
    • PLC 148 antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • PLCG 1 antibody
    • PLCG 1 antibody
    • PLCG1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    • PLCII antibody
    • triphosphoinositide phosphodiesterase antibody
    see all

    Database links

    see all

    Anti-Phospholipase C gamma 1 antibody [2B1] images:

      Western blot - Phospholipase C gamma antibody [2B1] (ab16955)

    Western blot - Phospholipase C gamma antibody [2B1] (ab16955)

    All lanes : Anti-Phospholipase C gamma 1 antibody [2B1] (ab16955)

    Lane 1 : HepG2 cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : Mouse brain


    Predicted band size : 149 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    References for Anti-Phospholipase C gamma 1 antibody [2B1] (ab16955)

    ab16955 has not yet been referenced specifically in any publications.

    Publishing research using ab16955? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"