![Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433) image](#ab41433_1.jpg)
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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Overview
Product name
Anti-Phospholipase C gamma 1 antibody [M156]
See all Phospholipase C gamma 1 products (18) ...
Description
Mouse monoclonal [M156] to Phospholipase C gamma 1
Specificity
Phospholipase C gamma 1 immunising peptide sequence has low homology to Phospholipase C gamma 2.
Tested applications
WB, ELISA, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to amino acids in the N-terminal region of human Phospholipase C gamma 1.
Positive control
Human A431, Hct116, and Jurkat cells; mouse brain.
General notes
Do not aliquot.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
M156
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
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Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)
![Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433) image](/ps/datasheet/Images/41/ab41433/ab41433_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab41433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
ELISA
ELISA: 1/2000ELISA: 1/2000
IP: Use at an assay dependent dilution. (5-10ul of antibody per 300-500 ug of total protein lysate.)
ICC/IF
ICC/IF: 1/100ICC/IF: 1/100
Target
Function
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Post-translational
modifications
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.
Ubiquitinated by CBLB in activated T-cells.
Target information above from: UniProt accessionP19174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate antibody
see all
Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all
Anti-Phospholipase C gamma 1 antibody [M156] images:
Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)
Predicted band size : 150 kDa
Western blot analysis of PLC gamma 1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-PLC gamma 1 (ab41433). The blots were probed with anti- PLC gamma 1 (lanes 1 & 2) and anti-PLC gamma 1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- PLC gamma 1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
References for Anti-Phospholipase C gamma 1 antibody [M156] (ab41433)
This product has been referenced in:
- Mizrachy-Schwartz Set al. Up-regulation of AMP-activated protein kinase in cancer cell lines is mediated through c-Src activation. J Biol Chem 286:15268-77 (2011). WB; Human.Read more (PubMed: 21245141) »
See 1 publication for this product
Publishing research using ab41433? Please let us know so that we can cite the reference in this datasheet
![Phospholipase C gamma 1 antibody [M156] for WB in Human (41433)](/ps/Reviews/Images/ab7830_33161.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"