Anti-Phospholipase C gamma 1 antibody [M156] (ab41433)
Overview
- Product nameAnti-Phospholipase C gamma 1 antibody [M156]See all Phospholipase C gamma 1 primary antibodies ...
- DescriptionMouse monoclonal [M156] to Phospholipase C gamma 1
- SpecificityPhospholipase C gamma 1 immunising peptide sequence has low homology to Phospholipase C gamma 2.
- Tested applicationsFlow Cyt, WB, ELISA, IP, ICC/IF more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat - Immunogen
Synthetic peptide corresponding to amino acids in the N-terminal region of human Phospholipase C gamma 1.
- Positive controlHuman A431, Hct116, and Jurkat cells; mouse brain.
- General notesDo not aliquot.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone numberM156
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab41433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt | Flow Cyt: 1/100. |
| WB | WB: 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
| ELISA | ELISA: 1/2000. |
| IP | IP: Use at an assay dependent dilution. 5-10ul of antibody per 300-500 ug of total protein lysate. |
| ICC/IF | ICC/IF: 1/100. |
Target
- FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
- Sequence similaritiesContains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. - DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
- Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. - Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
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Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
- Unigene: 44463 Mouse
- Unigene: 11243 Rat
see all
Target information above from: UniProt accession
P19174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody1 phosphatidyl D myo inositol 4 5 bisphosphate antibody1 phosphatidylinositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate antibody1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibodyInositoltrisphosphohydrolase antibodyInositoltrisphosphohydrolase antibodyMonophosphatidylinositol phosphodiesterase antibodyMonophosphatidylinositol phosphodiesterase antibodyNCKAP3 antibodyPhosphatidylinositol phospholipase C antibodyPhosphatidylinositol phospholipase C antibodyPhosphodiesterase gamma 1 antibodyPhosphodiesterase gamma 1 antibodyPhosphoinositidase C antibodyPhosphoinositidase C antibodyPhosphoinositide phospholipase C antibodyPhosphoinositide phospholipase C antibodyPhosphoinositide phospholipase C-gamma-1 antibodyPhospholipase C 148 antibodyPhospholipase C 148 antibodyPhospholipase C gamma 1 antibodyPhospholipase C gamma 1 antibodyPhospholipase C-gamma-1 antibodyPhospholipase C-II antibodyPLC 1 antibodyPLC 148 antibodyPLC 148 antibodyPLC gamma 1 antibodyPLC gamma 1 antibodyPLC II antibodyPLC II antibodyPLC-148 antibodyPLC-gamma-1 antibodyPLC-II antibodyPLC1 antibodyPLC148 antibodyPLCG 1 antibodyPLCG 1 antibodyPLCG1 antibodyPLCG1_HUMAN antibodyPLCgamma1 antibodyPLCII antibodyTriphosphoinositide phosphodiesterase antibodyTriphosphoinositide phosphodiesterase antibody
see all
Anti-Phospholipase C gamma 1 antibody [M156] images
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Predicted band size : 150 kDa -
Overlay histogram showing Jurkat cells stained with ab41433 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41433, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-Phospholipase C gamma 1 antibody [M156] (ab41433)
This product has been referenced in:
- Mizrachy-Schwartz S et al. Up-regulation of AMP-activated protein kinase in cancer cell lines is mediated through c-Src activation. J Biol Chem 286:15268-77 (2011). WB ; Human . Read more (PubMed: 21245141) »
![Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433) Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)](http://a.abcam.com/ps/datasheet/Images/41/ab41433/ab41433_1.jpg)
 Flow Cytometry-Anti-Phospholipase C gamma 1 antibody [M156](ab41433)](http://a.abcam.com/ps/datasheet/images/41/ab41433/Phospholipase-C-gamma-1-Primary-antibodies-ab41433-1.jpg)