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Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)
![Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)](/ps/datasheet/images/ab41433_1.jpg)
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Product Name
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Phospholipase C gamma 1 antibody [M156]
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See all Phospholipase C gamma 1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [M156] to Phospholipase C gamma 1
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Immunogen
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Synthetic peptide corresponding to amino acids in the N-terminal region of human Phospholipase C gamma 1.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat (Due to sequence homology)
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Specificity
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Phospholipase C gamma 1 immunising peptide sequence has low homology to Phospholipase C gamma 2.
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Tested applications
(see key)
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ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/2000. ICC/IF: 1/100. IP: 5-10ul of antibody per 300-500 ug of total protein lysate. WB: 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human A431, Hct116, and Jurkat cells; mouse brain.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins Signal Transduction >> Metabolism >> Lipid metabolism Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
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Relevance
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Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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M156
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at -20°C, Stable for 6 months at -20°C
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Notes
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Do not aliquot.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Phospholipase C gamma 1 antibody [M156] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Phospholipase C gamma 1 antibody [M156] images, references, products related to ab41433 and other tools.
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Phospholipase C gamma 1 antibody [M156] images:
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Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)
Predicted band size : 150 kDa
Western blot analysis of PLC gamma 1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-PLC gamma 1 (ab41433). The blots were probed with anti- PLC gamma 1 (lanes 1 & 2) and anti-PLC gamma 1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- PLC gamma 1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
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Search PubMed (MEDLINE) for references to Phospholipase C gamma 1
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