|
|
|
|
Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)
![Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)](/ps/datasheet/images/ab41433_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Phospholipase C gamma 1 antibody [M156]
|
| |
See all Phospholipase C gamma 1 antibodies (14)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [M156] to Phospholipase C gamma 1
|
|
Immunogen
|
Synthetic peptide corresponding to amino acids in the N-terminal region of human Phospholipase C gamma 1.
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Rat
(Due to sequence homology)
|
|
Specificity
|
Phospholipase C gamma 1 immunising peptide sequence has low homology to Phospholipase C gamma 2.
|
|
Tested applications
(see key)
|
ELISA, ICC/IF, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/2000.
ICC/IF: 1/100.
IP: 5-10ul of antibody per 300-500 ug of total protein lysate.
WB: 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human A431, Hct116, and Jurkat cells; mouse brain.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
|
|
Relevance
|
Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
M156
|
|
Isotype
|
IgG1
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Storage instructions
|
Store at -20°C, Stable for 6 months at -20°C
|
|
Notes
|
Do not aliquot.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Phospholipase C gamma 1 antibody [M156] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Phospholipase C gamma 1 antibody [M156] images, references, products related to ab41433 and other tools.
|
| |
Phospholipase C gamma 1 antibody [M156] images:
|
 Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)
Predicted band size : 150 kDa
Western blot analysis of PLC gamma 1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-PLC gamma 1 (ab41433). The blots were probed with anti- PLC gamma 1 (lanes 1 & 2) and anti-PLC gamma 1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- PLC gamma 1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
|
| |
| |
|
Search PubMed (MEDLINE) for references to Phospholipase C gamma 1
|
| |
Phospholipase C gamma 1 antibody [M156] - more information
|
Customer reviews (feedback) regarding Phospholipase C gamma 1 antibody [M156] |
Customer FAQs regarding Phospholipase C gamma 1 antibody [M156] |
Protocols for Phospholipase C gamma 1 antibody [M156] |
Price and availability of products related to Phospholipase C gamma 1 antibody [M156] |
| |
Phospholipase C gamma 1 antibody [M156] - related products:
|
|
|
| |
Phospholipase C gamma 1 antibody [M156] - other tools:
|
Contact Abcam with a Technical Enquiry about Phospholipase C gamma 1 antibody [M156] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)](/index.html?datasheet=41433#ab41433_1.jpg)
