Anti-Phospholipase C gamma 1 antibody [M156] (ab41433)

Overview

• Product nameAnti-Phospholipase C gamma 1 antibody [M156]See all Phospholipase C gamma 1 primary antibodies ...
• Description
  Mouse monoclonal [M156] to Phospholipase C gamma 1
• SpecificityPhospholipase C gamma 1 immunising peptide sequence has low homology to Phospholipase C gamma 2.
• Tested applicationsFlow Cyt, WB, ELISA, IP, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to amino acids in the N-terminal region of human Phospholipase C gamma 1.

• Positive controlHuman A431, Hct116, and Jurkat cells; mouse brain.
• General notesDo not aliquot.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS, 1mg/ml BSA
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberM156
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Hydrolysis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • PLC

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Hydrolysis

Applications

Target

• FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
• Sequence similaritiesContains 1 C2 domain.
  Contains 1 EF-hand domain.
  Contains 2 PH domains.
  Contains 1 PI-PLC X-box domain.
  Contains 1 PI-PLC Y-box domain.
  Contains 2 SH2 domains.
  Contains 1 SH3 domain.
• DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
• Post-translational
  modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
  Ubiquitinated by CBLB in activated T-cells.
• Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.

Target information above from: UniProt accession P19174 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5335 Human
  • Entrez Gene: 18803 Mouse
  • Entrez Gene: 25738 Rat
  • Omim: 172420 Human
  • SwissProt: P19174 Human
  • SwissProt: Q62077 Mouse
  • SwissProt: P10686 Rat
  • Unigene: 268177 Human

  • Unigene: 44463 Mouse
  • Unigene: 11243 Rat

  see all

• Alternative names
  1 phosphatidyl D myo inositol 4 5 bisphosphate antibody1 phosphatidyl D myo inositol 4 5 bisphosphate antibody1 phosphatidylinositol 4 5 bisphosphate antibody

  1 phosphatidylinositol 4 5 bisphosphate antibody1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibodyInositoltrisphosphohydrolase antibodyInositoltrisphosphohydrolase antibodyMonophosphatidylinositol phosphodiesterase antibodyMonophosphatidylinositol phosphodiesterase antibodyNCKAP3 antibodyPhosphatidylinositol phospholipase C antibodyPhosphatidylinositol phospholipase C antibodyPhosphodiesterase gamma 1 antibodyPhosphodiesterase gamma 1 antibodyPhosphoinositidase C antibodyPhosphoinositidase C antibodyPhosphoinositide phospholipase C antibodyPhosphoinositide phospholipase C antibodyPhosphoinositide phospholipase C-gamma-1 antibodyPhospholipase C 148 antibodyPhospholipase C 148 antibodyPhospholipase C gamma 1 antibodyPhospholipase C gamma 1 antibodyPhospholipase C-gamma-1 antibodyPhospholipase C-II antibodyPLC 1 antibodyPLC 148 antibodyPLC 148 antibodyPLC gamma 1 antibodyPLC gamma 1 antibodyPLC II antibodyPLC II antibodyPLC-148 antibodyPLC-gamma-1 antibodyPLC-II antibodyPLC1 antibodyPLC148 antibodyPLCG 1 antibodyPLCG 1 antibodyPLCG1 antibodyPLCG1_HUMAN antibodyPLCgamma1 antibodyPLCII antibodyTriphosphoinositide phosphodiesterase antibodyTriphosphoinositide phosphodiesterase antibody

  see all

Anti-Phospholipase C gamma 1 antibody [M156] images

• 

  Western blot - Phospholipase C gamma 1 antibody [M156] (ab41433)
  
  
  Predicted band size : 150 kDa
  
• 

  Flow Cytometry-Anti-Phospholipase C gamma 1 antibody [M156](ab41433)
  Overlay histogram showing Jurkat cells stained with ab41433 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41433, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.