Anti-Phospholipase C gamma 1 (phospho Y771) antibody (ab52207)

Overview

  • Product name
    Anti-Phospholipase C gamma 1 (phospho Y771) antibody
    See all Phospholipase C gamma 1 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase C gamma 1 (phospho Y771)
  • Specificity
    ab52207 detects endogenous levels of PLCG1 only when phosphorylated at tyrosine 771.
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human PLCG1 around the phosphorylation site of tyrosine 771 (P-D-YP-G-A).

  • Positive control
    • Human breast carcinoma tissue; extracts from COS7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab52207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 175 kDa (predicted molecular weight: 149 kDa).
ELISA 1/40000.

Target

  • Function
    Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • Post-translational
    modifications
    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • Cellular localization
    Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • Phosphatidylinositol phospholipase C antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • Plcg1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    see all

Images

  • This image shows human breast carcinoma tissue stained with ab52207 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
  • All lanes : Anti-Phospholipase C gamma 1 (phospho Y771) antibody (ab52207) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated with EGF (200ng/ml, 30min) with no immunising peptide
    Lane 2 : Extracts from COS7 cells treated with EGF (200ng/ml, 30min) with immunising peptide


    Predicted band size : 149 kDa
    Observed band size : ~175 kDa (why is the actual band size different from the predicted?)

References

ab52207 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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