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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab4828? Please let us know so that we can cite the reference in this datasheet
ab4828 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Phospholipase C gamma 1 (phospho Y783) antibody (ab4828)
Predicted band size : 135 kDa
Extracts prepared from NIH-3T3 cells exposed to PDGF (10 ng/mL) for 10 minutes were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 µg/mL ab4828 antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix WesternStar detection method and Kodak BioMax ultraclear film. The data show that PDGF is a strong inducer of PLC gamma 1 phosphorylation on Y783 in NIH3T3 cells.
ab5163 at a diluton of 1/1000 staining SLPI in formalin fixed paraffin embedded sections of a) inflammed human gastric epithelium and b) normal human gastric epithelium by immunohistochemistry.
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