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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Overview
Product name
Anti-Phospholipase C gamma 1 (phospho Y783) antibody
See all Phospholipase C gamma 1 products (18) ...
Description
Rabbit polyclonal to Phospholipase C gamma 1 (phospho Y783)
Specificity
Phosphorylation site-specific antibody selective for the phosphorylated form of the Phospholipase C gamma 1 containing a phosphate on tyrosine 783. The antibody has been shown to recognize the endogenous form of Phospholipase C gamma when phosphorylated on tyrosine 783 in a variety of cell types following treatment by a broad range of extracellular stimuli. Applications include NIH 3T3 cells +/- PDGF. The antibody does not react with a site directed mutant (Y783F).
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Cow, Xenopus laevis, a wide range of other species
Immunogen
Synthetic peptide (Human) derived from the region of Phospholipase C gamma that contains tyrosine 783, based on the human sequence. This region is conserved among many species including human, rat, bovine and frog (Xenopus laevis).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with nonphosphorylated Phospholipase C gamma enzymes and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an Phospholipase C gamma-derived peptide that is phosphorylated at tyrosine 783.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 135 kDa. Can be blocked with Phospholipase C gamma 1 phospho Y783 and non-phospho peptides (pair)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Post-translational
modifications
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.
Ubiquitinated by CBLB in activated T-cells.
Target information above from: UniProt accessionP19174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
see all
Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P08487 Cow
- SwissProt: P19174 Human
- SwissProt: P10686 Rat
- Unigene: 268177 Human
- Unigene: 11243 Rat
Anti-Phospholipase C gamma 1 (phospho Y783) antibody images:
Western blot - Phospholipase C gamma 1 (phospho Y783) antibody (ab4828)
Predicted band size : 135 kDa
Extracts prepared from NIH-3T3 cells exposed to PDGF (10 ng/mL) for 10 minutes were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 µg/mL ab4828 antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix WesternStar detection method and Kodak BioMax ultraclear film. The data show that PDGF is a strong inducer of PLC gamma 1 phosphorylation on Y783 in NIH3T3 cells.
ab5163 at a diluton of 1/1000 staining SLPI in formalin fixed paraffin embedded sections of a) inflammed human gastric epithelium and b) normal human gastric epithelium by immunohistochemistry.
References for Anti-Phospholipase C gamma 1 (phospho Y783) antibody (ab4828)
ab4828 has not yet been referenced specifically in any publications.
Publishing research using ab4828? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"