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Western blot - Phospholipase C gamma 1 (phospho Y783) antibody (ab4828)
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Product Name
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Phospholipase C gamma 1 (phospho Y783) antibody
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See all Phospholipase C gamma 1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Phospholipase C gamma 1 (phospho Y783)
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Immunogen
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Synthetic peptide (Human) derived from the region of Phospholipase C gamma that contains tyrosine 783, based on the human sequence. This region is conserved among many species including human, rat, bovine and frog (Xenopus laevis).
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Reacts with
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Hu
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Predicted to react
(species key)
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Rat, Many, Cow, Xl
(Due to sequence homology)
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Specificity
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Phosphorylation site-specific antibody selective for the phosphorylated form of the Phospholipase C gamma 1 containing a phosphate on tyrosine 783. The antibody has been shown to recognize the endogenous form of Phospholipase C gamma when phosphorylated on tyrosine 783 in a variety of cell types following treatment by a broad range of extracellular stimuli. Applications include NIH 3T3 cells +/- PDGF. The antibody does not react with a site directed mutant (Y783F).
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 135 kDa. Can be blocked with Phospholipase C gamma 1 phospho Y783 and non-phospho peptides (pair)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
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Relevance
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Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with nonphosphorylated Phospholipase C gamma enzymes and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an Phospholipase C gamma-derived peptide that is phosphorylated at tyrosine 783.
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Phospholipase C gamma 1 (phospho Y783) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Phospholipase C gamma 1 (phospho Y783) antibody images, references, products related to ab4828 and other tools.
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Phospholipase C gamma 1 (phospho Y783) antibody images:
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 Western blot - Phospholipase C gamma 1 (phospho Y783) antibody (ab4828)
Predicted band size : 135 kDa
Extracts prepared from NIH-3T3 cells exposed to PDGF (10 ng/mL) for 10 minutes were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 µg/mL ab4828 antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix WesternStar detection method and Kodak BioMax ultraclear film. The data show that PDGF is a strong inducer of PLC gamma 1 phosphorylation on Y783 in NIH3T3 cells.
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ab5163 at a diluton of 1/1000 staining SLPI in formalin fixed paraffin embedded sections of a) inflammed human gastric epithelium and b) normal human gastric epithelium by immunohistochemistry.
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Search PubMed (MEDLINE) for references to Phospholipase C gamma 1
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Phospholipase C gamma 1 (phospho Y783) antibody - more information
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