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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Overview
Product name
Anti-Phospholipase C gamma 1 (phospho Y783) antibody
See all Phospholipase C gamma 1 products (18) ...
Description
Rabbit polyclonal to Phospholipase C gamma 1 (phospho Y783)
Specificity
ab53125 detects endogenous levels of Phospholipase C gamma 1 only when phosphorylated at tyrosine 783.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthetic phosphopeptide derived from human Phospholipase C gamma 1 around the phosphorylation site of tyrosine 783 (G-F-YP-V-E).
Positive control
Human breast carcinoma tissue and cos7 cell extracts treated with EGF (200ng/ml, 30mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53125 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000.Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Post-translational
modifications
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.
Ubiquitinated by CBLB in activated T-cells.
Target information above from: UniProt accessionP19174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
see all
Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all
Anti-Phospholipase C gamma 1 (phospho Y783) antibody images:
Western blot - Phospholipase C gamma 1 (phospho Y783) antibody (ab53125)
All lanes : Anti-Phospholipase C gamma 1 (phospho Y783) antibody (ab53125) at 1/300 dilution
Lane 1 : cos7 cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : cos7 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size : 149 kDa
Immunohistochemistry (Paraffin-embedded sections) - Phospholipase C gamma 1 (phospho Y783) antibody (ab53125)
ab53125 at 1/50 dilution staining Phospholipase C gamma 1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
References for Anti-Phospholipase C gamma 1 (phospho Y783) antibody (ab53125)
ab53125 has not yet been referenced specifically in any publications.
Publishing research using ab53125? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"