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Products:Signal Transduction >> Metabolism >> Amino Acids
Anti-Phosphoserine antibody [3C171] (ab17465)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab17465? Please let us know so that we can cite the reference in this datasheet
ab17465 has been referenced in 3 publications.
- Almeida S et al. Dysregulation of CREB activation and histone acetylation in 3-nitropropionic acid-treated cortical neurons: prevention by BDNF and NGF. Neurotox Res 17:399-405 (2010). WB; Rat. PubMed: 19779956
- Lee Y et al. Negative feedback Inhibition of NFATc1 by DYRK1A regulates bone homeostasis. J Biol Chem 284:33343-51 (2009). WB; Mouse. PubMed: 19801542
- Allard D et al. Akt Regulates the Survival of Vascular Smooth Muscle Cells via Inhibition of FoxO3a and GSK3. J Biol Chem 283:19739-47 (2008). WB; Rat. PubMed: 18458087
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
](/ps/datasheet/images/17/ab17465/Phosphoserine-Primary-antibodies-ab17465-1.jpg)
Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465)
ICC/IF image of ab17465 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17465, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Western blot - Phosphoserine antibody [3C171] (ab17465)](/ps/datasheet/images/17/ab17465/Phosphoserine-Primary-antibodies-ab17465-3.jpg)
Western blot - Phosphoserine antibody [3C171] (ab17465)
Western blot of canine brain hippocampus homogenates (10 ug loaded per lane for 3 different samples) separated on SDS-PAGE and blotted with ab17465.
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