Phosphoserine antibody [3C171] (ab17465)

Product Name 

Phosphoserine antibody [3C171]

Product type 

Primary antibodies

Description 

Mouse monoclonal [3C171] to Phosphoserine

Immunogen 

Phosphoserine conjugated to KLH.

Reacts with 
(species key)

Hu, Rat, Chk, Cow, Dog, Goose, Hm, Mk

Specificity 

Reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.

Tested applications 
(see key)

ELISA, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/500.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250 (colorimetric).

Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3C171

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 0.05% Sodium Azide, 0.01% Thimerosal.
Constituents: 40% Glycerol, PBS, 15mg/ml BSA. pH 7.6

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

2.600 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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References for Phosphoserine antibody [3C171] (ab17465)

  This product has been used in: (two most recent references)

Lee Y et al. Negative feedback Inhibition of NFATc1 by DYRK1A regulates bone homeostasis. J Biol Chem 284:33343-51 (2009). WB; Mouse. PubMed: 19801542

Allard D et al. Akt Regulates the Survival of Vascular Smooth Muscle Cells via Inhibition of FoxO3a and GSK3. J Biol Chem 283:19739-47 (2008). WB; Rat. PubMed: 18458087
If you publish research using ab17465 please let us know so that we can cite the reference on this datasheet.

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