 image](#Phosphoserine-Primary-antibodies-ab17465-1.jpg)
![Western blot - Phosphoserine antibody [3C171] (ab17465) image](#Phosphoserine-Primary-antibodies-ab17465-3.jpg)
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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Phosphoserine antibody [3C171]
See all Phosphoserine products (8) ...
Description
Mouse monoclonal [3C171] to Phosphoserine
Specificity
Reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.
Tested applications
ELISA, WB, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Chicken, Hamster, Cow, Dog, Human, Monkey, Goose
Immunogen
Phosphoserine conjugated to KLH.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide, 0.01% Thimerosal.
Constituents: 40% Glycerol, PBS, 15mg/ml BSA. pH 7.6
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
3C171
Isotype
IgG1
Research areas
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/500ELISA: 1/500
WB: 1/250((colorimetric). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.)
ICC/IF: Use a concentration of 1 µg/ml
Target
Relevance
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Alternative names
- phospho-Ser antibody
- pSER antibody
Anti-Phosphoserine antibody [3C171] images:
Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465)
](/ps/datasheet/images/17/ab17465/Phosphoserine-Primary-antibodies-ab17465-1.jpg)
ICC/IF image of ab17465 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17465, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Phosphoserine antibody [3C171] (ab17465)
![Western blot - Phosphoserine antibody [3C171] (ab17465)](/ps/datasheet/images/17/ab17465/Phosphoserine-Primary-antibodies-ab17465-3.jpg)
Western blot of canine brain hippocampus homogenates (10 ug loaded per lane for 3 different samples) separated on SDS-PAGE and blotted with ab17465.
References for Anti-Phosphoserine antibody [3C171] (ab17465)
This product has been referenced in:
- Almeida Set al. Dysregulation of CREB activation and histone acetylation in 3-nitropropionic acid-treated cortical neurons: prevention by BDNF and NGF. Neurotox Res 17:399-405 (2010). WB; Rat.Read more (PubMed: 19779956) »
- Lee Yet al. Negative feedback Inhibition of NFATc1 by DYRK1A regulates bone homeostasis. J Biol Chem 284:33343-51 (2009). WB; Mouse.Read more (PubMed: 19801542) »
See all 3 publications for this product
Publishing research using ab17465? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"