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Anti-Phosphoserine antibody [3C171] (ab17465)

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Overview

Product name

Anti-Phosphoserine antibody [3C171]
See all Phosphoserine products (8) ...

Description

Mouse monoclonal [3C171] to Phosphoserine

Specificity

Reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.

Tested applications

ELISA, WB, ICC/IFmore details

Cross reactivity

Reacts with

Rat, Chicken, Hamster, Cow, Dog, Human, Monkey, Goose

Immunogen

Phosphoserine conjugated to KLH.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide, 0.01% Thimerosal.
Constituents: 40% Glycerol, PBS, 15mg/ml BSA. pH 7.6

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

3C171

Isotype

IgG1

  • Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465)Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465) image (enlarge)

  • Western blot - Phosphoserine antibody [3C171] (ab17465)Western blot - Phosphoserine antibody [3C171] (ab17465) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Alternative names

  • phospho-Ser antibody
  • pSER antibody

Anti-Phosphoserine antibody [3C171] images:

  Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465)

Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465)

ICC/IF image of ab17465 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17465, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Western blot - Phosphoserine antibody [3C171] (ab17465)

Western blot - Phosphoserine antibody [3C171] (ab17465)

Western blot of canine brain hippocampus homogenates (10 ug loaded per lane for 3 different samples) separated on SDS-PAGE and blotted with ab17465.

References for Anti-Phosphoserine antibody [3C171] (ab17465)

This product has been referenced in:

  • Almeida Set al. Dysregulation of CREB activation and histone acetylation in 3-nitropropionic acid-treated cortical neurons: prevention by BDNF and NGF. Neurotox Res 17:399-405 (2010). WB; Rat.Read more (PubMed: 19779956) »
  • Lee Yet al. Negative feedback Inhibition of NFATc1 by DYRK1A regulates bone homeostasis. J Biol Chem 284:33343-51 (2009). WB; Mouse.Read more (PubMed: 19801542) »

See all 3 publications for this product

Publishing research using ab17465? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"