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Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465)
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Product Name
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Phosphoserine antibody [3C171]
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See all Phosphoserine antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3C171] to Phosphoserine
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Immunogen
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Phosphoserine conjugated to KLH.
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Reacts with
(species key)
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Hu, Rat, Chk, Cow, Dog, Goose, Hm, Mk
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Specificity
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Reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/500.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250 (colorimetric).
Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3C171
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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Preservative: 0.05% Sodium Azide, 0.01% Thimerosal.
Constituents: 40% Glycerol, PBS, 15mg/ml BSA. pH 7.6 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.600 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Phosphoserine antibody [3C171] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Phosphoserine antibody [3C171] images, references, products related to ab17465 and other tools.
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Phosphoserine antibody [3C171] images:
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 Immunocytochemistry/ Immunofluorescence-Phosphoserine antibody [3C171](ab17465)
ICC/IF image of ab17465 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17465, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Phosphoserine antibody [3C171] (ab17465)
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This product has been used in: (two most recent references)
Lee Y et al. Negative feedback Inhibition of NFATc1 by DYRK1A regulates bone homeostasis. J Biol Chem 284:33343-51 (2009). WB; Mouse. PubMed: 19801542
Allard D et al. Akt Regulates the Survival of Vascular Smooth Muscle Cells via Inhibition of FoxO3a and GSK3. J Biol Chem 283:19739-47 (2008). WB; Rat. PubMed: 18458087
If you publish research using ab17465 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Phosphoserine
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Phosphoserine antibody [3C171] - more information
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Customer reviews (feedback) regarding Phosphoserine antibody [3C171] |
Customer FAQs regarding Phosphoserine antibody [3C171] |
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Price and availability of products related to Phosphoserine antibody [3C171] |
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Phosphoserine antibody [3C171] - related products:
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Phosphoserine antibody [3C171] - other tools:
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Contact Abcam with a Technical Enquiry about Phosphoserine antibody [3C171] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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