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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Phosphoserine antibody (HRP)
See all Phosphoserine products (8) ...
Description
Rabbit polyclonal to Phosphoserine (HRP)
Conjugation
HRP
Conjugation notes
HRP
Specificity
Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
Tested applications
WB, ELISAmore details
Immunogen
BSA and KLH-phosphoserine conjugates.
Positive control
Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Immunoaffinity chromatography with phosphoserine-agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids
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Western blot - Phosphoserine antibody (HRP) (ab9334)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/ml(To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.)
ELISA: Use a concentration of 1 µg/ml
Target
Relevance
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Alternative names
- phospho-Ser antibody
- pSER antibody
Anti-Phosphoserine antibody (HRP) images:
Western blot - Phosphoserine antibody (HRP) (ab9334)
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 5 minutes
Western blotting of mouse brain extract with ab9334 at a 1/200 dilution on a 12% SDS gel. 50 µg protein loaded in each lane. Antibody incubated for 2 hours incubation at room temperature.
References for Anti-Phosphoserine antibody (HRP) (ab9334)
This product has been referenced in:
- Martínez-Florensa Met al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB; Human.Read more (PubMed: 20940326) »
- Zang ZJet al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB.Read more (PubMed: 18940248) »
See all 8 publications for this product
Publishing research using ab9334? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"