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Western blot - Phosphoserine antibody (HRP) (ab9334)

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Product Name
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Phosphoserine antibody (HRP)
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See all Phosphoserine antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Phosphoserine (HRP)
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Immunogen
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BSA and KLH-phosphoserine conjugates.
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Specificity
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Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at a concentration of 1 µg/ml. WB: Use at a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
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Research areas
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Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation Signal Transduction >> Protein Phosphorylation >> pSer / pThr Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Conjugation notes
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HRP
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Storage buffer
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Tris buffered saline
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Purification notes
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Immunoaffinity chromatography with phosphoserine-agarose.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Phosphoserine antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Phosphoserine antibody (HRP) images, references, products related to ab9334 and other tools.
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Phosphoserine antibody (HRP) images:
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Western blot - Phosphoserine antibody (HRP) (ab9334)
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 5 minutes
Western blotting of mouse brain extract with ab9334 at a 1/200 dilution on a 12% SDS gel. 50 µg protein loaded in each lane. Antibody incubated for 2 hours incubation at room temperature.
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References for Phosphoserine antibody (HRP) (ab9334)
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This product has been used in: (two most recent references)
Zang ZJ et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB. PubMed: 18940248
Selvarajoo K et al. Signaling flux redistribution at toll-like receptor pathway junctions. PLoS One 3:e3430 (2008). WB. PubMed: 18927610
See all 7 publication references for this product.
If you publish research using ab9334 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Phosphoserine
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Phosphoserine antibody (HRP) - more information
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Customer reviews (feedback) regarding Phosphoserine antibody (HRP) |
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Protocols for Phosphoserine antibody (HRP) |
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Phosphoserine antibody (HRP) - related products:
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Phosphoserine antibody (HRP) - other tools:
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Contact Abcam with a Technical Enquiry about Phosphoserine antibody (HRP) |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"