Phosphoserine antibody (HRP) (ab9334)

Product Name 

Phosphoserine antibody (HRP)

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Phosphoserine (HRP)

Immunogen 

BSA and KLH-phosphoserine conjugates.

Specificity 

Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins.
Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine.
Has slight cross-reactivity with free phosphothreonine.
Readily reacts to known phosphoproteins such as phosvitin and alpha casein.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 2 µg/ml.
To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Conjugation notes 

HRP

Storage buffer 

Tris buffered saline

Purification notes 

Immunoaffinity chromatography with phosphoserine-agarose.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for Phosphoserine antibody (HRP) images, references, products related to ab9334 and other tools.

Phosphoserine antibody (HRP) images:

References for Phosphoserine antibody (HRP) (ab9334)

  This product has been used in: (two most recent references)

Zang ZJ et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB. PubMed: 18940248

Selvarajoo K et al. Signaling flux redistribution at toll-like receptor pathway junctions. PLoS One 3:e3430 (2008). WB. PubMed: 18927610

See all 7 publication references for this product.


If you publish research using ab9334 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Phosphoserine antibody (HRP)

Customer FAQs regarding Phosphoserine antibody (HRP)

Protocols for Phosphoserine antibody (HRP)

Price and availability of products related to Phosphoserine antibody (HRP)

Phosphoserine antibody (HRP) - related products: